TNAP Antibody (TRA-2-39): sc-21708
- TNAP Antibody (TRA-2-39) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- raised against 2102Ep human embryonal carcinoma cells
- TNAP Antibody (TRA-2-39) is recommended for detection of TNAP of human origin by WB, IP, IF, IHC(P) and FCM
- TNAP Antibody (TRA-2-39) is available conjugated to agarose for IP; and to HRP for WB, IHC(P) and ELISA
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TNAP Antibody (TRA-2-39) for WB and IHC(P) applications. These reagents are now offered in bundles with TNAP Antibody (TRA-2-39) (see ordering information below).
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TNAP Antibody (TRA-2-39) is a mouse monoclonal IgG1 kappa light chain antibody that detects TNAP of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). TNAP (TRA-2-39) antibody is available in both conjugated and non-conjugated forms, providing versatility for various experimental needs. The tissue non-specific alkaline phosphatase (TNAP) is a crucial enzyme that plays a significant role in bone mineralization, functioning alongside plasma cell membrane glycoprotein-1. This enzyme catalyzes the hydrolysis of phospho-monoesters into inorganic phosphate and alcohol, a process vital for maintaining phosphate homeostasis in the body. TNAP is widely expressed in several tissues, including the liver, kidney, bone, stomach, and colon, and is encoded by a gene located on chromosome 1p36.12. Notably, while TNAP is essential for normal bone mineralization, studies have shown that mice lacking functional TNAP can still exhibit normal skeletal development, indicating the presence of compensatory mechanisms. However, mutations in the TNAP gene are directly linked to hypophosphatasia, a rare genetic disorder characterized by defective bone mineralization and associated skeletal defects. Understanding TNAP′s function and interactions with other proteins is critical for elucidating the underlying mechanisms of bone-related diseases and developing potential therapeutic strategies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TNAP Antibody (TRA-2-39) References:
- TNAP activity is localized at critical sites of retinal neurotransmission across various vertebrate species. | Kántor, O., et al. 2014. Cell Tissue Res. 358: 85-98. PMID: 24988913
- Counter-regulatory phosphatases TNAP and NPP1 temporally regulate tooth root cementogenesis. | Zweifler, LE., et al. 2015. Int J Oral Sci. 7: 27-41. PMID: 25504209
- Effects of GPI-anchored TNAP on the dynamic structure of model membranes. | Garcia, AF., et al. 2015. Phys Chem Chem Phys. 17: 26295-301. PMID: 26389140
- Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators. | Simão, AMS., et al. 2019. J Bone Miner Metab. 37: 607-613. PMID: 30324534
- Loss of tissue-nonspecific alkaline phosphatase (TNAP) enzyme activity in cerebral microvessels is coupled to persistent neuroinflammation and behavioral deficits in late sepsis. | Nwafor, DC., et al. 2020. Brain Behav Immun. 84: 115-131. PMID: 31778743
- TNAP inhibition attenuates cardiac fibrosis induced by myocardial infarction through deactivating TGF-β1/Smads and activating P53 signaling pathways. | Gao, L., et al. 2020. Cell Death Dis. 11: 44. PMID: 31969558
- Tissue-Nonspecific Alkaline Phosphatase (TNAP) as the Enzyme Involved in the Degradation of Nucleotide Analogues in the Ligand Docking and Molecular Dynamics Approaches. | Madaj, R., et al. 2021. Biomolecules. 11: PMID: 34439771
- TNAP upregulation is a critical factor in Tauopathies and its blockade ameliorates neurotoxicity and increases life-expectancy. | Sebastián-Serrano, Á., et al. 2022. Neurobiol Dis. 165: 105632. PMID: 35065251
- Promotion effect of FGF23 on osteopenia in congenital scoliosis through FGFr3/TNAP/OPN pathway. | Zhang, H., et al. 2023. Chin Med J (Engl). 136: 1468-1477. PMID: 37192015
- Dentin Matrix Protein 1 Regulates Mineralization of MC3T3-E1 Cells via the TNAP-ANK-ENPP1 Axis. | Liu, J., et al. 2023. J Nippon Med Sch. 90: 262-271. PMID: 37380477
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TNAP Antibody (TRA-2-39) | sc-21708 | 200 µg/ml | $316.00 | |||
TNAP Antibody (TRA-2-39): m-IgG Fc BP-HRP Bundle | sc-528360 | 200 µg Ab; 10 µg BP | $354.00 | |||
TNAP Antibody (TRA-2-39): m-IgGκ BP-HRP Bundle | sc-520720 | 200 µg Ab, 40 µg BP | $354.00 | |||
TNAP Antibody (TRA-2-39): m-IgG1 BP-HRP Bundle | sc-542891 | 200 µg Ab; 20 µg BP | $354.00 | |||
TNAP Antibody (TRA-2-39) AC | sc-21708 AC | 500 µg/ml, 25% agarose | $416.00 | |||
TNAP Antibody (TRA-2-39) HRP | sc-21708 HRP | 200 µg/ml | $316.00 | |||
TNAP Antibody (TRA-2-39) Alexa Fluor® 488 | sc-21708 AF488 | 200 µg/ml | $357.00 | |||
TNAP Antibody (TRA-2-39) Alexa Fluor® 546 | sc-21708 AF546 | 200 µg/ml | $357.00 | |||
TNAP Antibody (TRA-2-39) Alexa Fluor® 594 | sc-21708 AF594 | 200 µg/ml | $357.00 | |||
TNAP Antibody (TRA-2-39) Alexa Fluor® 647 | sc-21708 AF647 | 200 µg/ml | $357.00 | |||
TNAP Antibody (TRA-2-39) Alexa Fluor® 680 | sc-21708 AF680 | 200 µg/ml | $357.00 | |||
TNAP Antibody (TRA-2-39) Alexa Fluor® 790 | sc-21708 AF790 | 200 µg/ml | $357.00 |