TLE3 Antibody (D-10): sc-514798

TLE3 Antibody (D-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects TLE3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TLE3 Antibody (D-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TLE3, also known as Enhancer of split groucho-like protein 3, plays a crucial role in the Notch signaling pathway, which regulates cellular interactions determining cell fate during development in both invertebrates and vertebrates. TLE3 (D-10) antibody targets a protein that functions as a transcriptional repressor, interacting with chromatin and specifically with Histone H3, thereby influencing gene expression patterns critical for processes such as neurogenesis and epithelial differentiation. Expression levels of TLE genes, including TLE3, are associated with immature epithelial cells transitioning toward a terminally differentiated state, highlighting their importance in cellular maturation. TLE3, along with TLE1 and TLE2, has been implicated in cervical squamous metaplasias and carcinomas, indicating potential relevance in cancer biology. Dynamic regulation of TLE gene expression during different stages of cell differentiation underscores their complex roles in development and disease, making anti-TLE3 antibody (D-10) a valuable research tool for studying these critical biological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TLE3 Antibody (D-10) References:

1. TCF and Groucho-related genes influence pituitary growth and development.  |  Brinkmeier, ML., et al. 2003. Mol Endocrinol. 17: 2152-61. PMID: 12907761
2. Human homologs of a Drosophila Enhancer of split gene product define a novel family of nuclear proteins.  |  Stifani, S., et al. 1992. Nat Genet. 2: 119-27. PMID: 1303260
3. Meningioma transcript profiles reveal deregulated Notch signaling pathway.  |  Cuevas, IC., et al. 2005. Cancer Res. 65: 5070-5. PMID: 15958550
4. Duplicated members of the Groucho/Tle gene family in fish.  |  Aghaallaei, N., et al. 2005. Dev Dyn. 234: 143-50. PMID: 16059907
5. TLE3 represses colorectal cancer proliferation by inhibiting MAPK and AKT signaling pathways.  |  Yang, RW., et al. 2016. J Exp Clin Cancer Res. 35: 152. PMID: 27669982
6. Differential functions of TLE1 and TLE3 depending on a specific phosphorylation site.  |  Kornspan, D., et al. 2021. Biochem Biophys Res Commun. 545: 164-170. PMID: 33571907
7. The transcriptional cofactor Tle3 reciprocally controls effector and central memory CD8+ T cell fates.  |  Zhao, X., et al. 2024. Nat Immunol. 25: 294-306. PMID: 38238608
8. Groucho is required for Drosophila neurogenesis, segmentation, and sex determination and interacts directly with hairy-related bHLH proteins.  |  Paroush, Z., et al. 1994. Cell. 79: 805-15. PMID: 8001118
9. Epithelial expression and chromosomal location of human TLE genes: implications for notch signaling and neoplasia.  |  Liu, Y., et al. 1996. Genomics. 31: 58-64. PMID: 8808280
10. The Groucho/transducin-like enhancer of split transcriptional repressors interact with the genetically defined amino-terminal silencing domain of histone H3.  |  Palaparti, A., et al. 1997. J Biol Chem. 272: 26604-10. PMID: 9334241
11. Combinatorial expression patterns of individual TLE proteins during cell determination and differentiation suggest non-redundant functions for mammalian homologs of Drosophila Groucho.  |  Yao, J., et al. 1998. Dev Growth Differ. 40: 133-46. PMID: 9572356
12. Transcriptional repression by AML1 and LEF-1 is mediated by the TLE/Groucho corepressors.  |  Levanon, D., et al. 1998. Proc Natl Acad Sci U S A. 95: 11590-5. PMID: 9751710

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TLE3 Antibody (D-10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TLE3 Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TLE3 Antibody (D-10) Specifications

Alternate Product Name: TLE3

Product Clone Name: D-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 200-400

Epitope Target: TLE3

Epitope Species: mouse

Product Reactivity/Detection: TLE3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TLE3 Antibody (D-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred TLE4 antibody is TLE4 Antibody (E-10): sc-365406.