TIP47 Antibody (E-5): sc-393461

TIP47 Antibody (E-5) is a mouse monoclonal IgG2a antibody that detects TIP47 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TIP47, also known as human placental tissue protein 17b (PP17b), plays a crucial role in intracellular protein transport by binding to cytoplasmic domains of cation-dependent and cation-independent mannose 6-phosphate receptors (MPRs), facilitating movement from endosomes to the Golgi complex. The selective binding of TIP47 to specific cargo molecules maintains proper protein sorting within the endosomal pathway, recognizing a phenylalanine/tryptophan signal sequence in cation-dependent MPR, essential for effective transport. TIP47′s interaction with cation-independent MPR depends on specific cytoplasmic residues, highlighting TIP47′s importance in protein trafficking regulation. TIP47 shows overexpression in tissue extracts from cervical carcinoma patients, with higher levels in dysplastic cells of high-grade dysplasias compared to low-grade dysplasias, suggesting potential use as a cancer progression biomarker. TIP47′s encoding gene location on chromosome 19pter-19p13.3 emphasizes significance in cellular processes and disease states.

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TIP47 Antibody (E-5) References:

1. Quantitative analysis of TIP47-receptor cytoplasmic domain interactions: implications for endosome-to-trans Golgi network trafficking.  |  Krise, JP., et al. 2000. J Biol Chem. 275: 25188-93. PMID: 10829017
2. Role of Rab9 GTPase in facilitating receptor recruitment by TIP47.  |  Carroll, KS., et al. 2001. Science. 292: 1373-6. PMID: 11359012
3. Targeting of the human immunodeficiency virus type 1 envelope to the trans-Golgi network through binding to TIP47 is required for env incorporation into virions and infectivity.  |  Blot, G., et al. 2003. J Virol. 77: 6931-45. PMID: 12768012
4. Spatial integration of TIP47 and adipophilin in macrophage lipid bodies.  |  Robenek, H., et al. 2005. J Biol Chem. 280: 5789-94. PMID: 15545278
5. Immunohistochemical staining for adipophilin, perilipin and TIP47.  |  Muthusamy, K., et al. 2006. J Clin Pathol. 59: 1166-70. PMID: 16556662
6. TIP47 is a key effector for Rab9 localization.  |  Aivazian, D., et al. 2006. J Cell Biol. 173: 917-26. PMID: 16769818
7. Functional compensation for adipose differentiation-related protein (ADFP) by Tip47 in an ADFP null embryonic cell line.  |  Sztalryd, C., et al. 2006. J Biol Chem. 281: 34341-8. PMID: 16968708
8. All-trans-retinol generated by rhodopsin photobleaching induces rapid recruitment of TIP47 to lipid droplets in the retinal pigment epithelium.  |  Tsuiki, E., et al. 2007. Invest Ophthalmol Vis Sci. 48: 2858-67. PMID: 17525222
9. Preliminary study of TIP47 as a possible new biomarker of cervical dysplasia and invasive carcinoma.  |  Szigeti, A., et al. 2009. Anticancer Res. 29: 717-24. PMID: 19331227
10. The cellular protein TIP47 restricts Respirovirus multiplication leading to decreased virus particle production.  |  Bampi, C., et al. 2013. Virus Res. 173: 354-63. PMID: 23348195

The preferred TIP47 antibody is TIP47 Antibody (B-3): sc-390981.