TIP47 Antibody (B-3): sc-390981

TIP47 Antibody (B-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects TIP47 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TIP47 (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The tail-interacting 47kDa protein (TIP47), also known as human placental tissue protein 17b (PP17b), plays a crucial role in intracellular protein transport by binding to cytoplasmic domains of cation-dependent and cation-independent mannose 6-phosphate receptors (MPRs), facilitating movement from endosomes to Golgi complex. This transport is vital for proper sorting and delivery of lysosomal enzymes, which are essential for cellular homeostasis and degradation of cellular waste. TIP47′s selective binding to specific cargo molecules highlights importance in maintaining efficiency of endosomal pathway. In cation-dependent MPR context, TIP47 recognizes a critical phenylalanine/tryptophan signal sequence necessary for accurate sorting, while for cation-independent MPR, specific cytoplasmic residues are required for high-affinity binding. TIP47 competes with AP-2 clathrin adaptor for binding, indicating significant role in regulation of endosomal trafficking. In clinical studies, TIP47 has been found overexpressed in tissue extracts from cervical carcinoma patients, with higher levels observed in dysplastic cells, suggesting potential as cancer progression biomarker. The gene encoding human TIP47 is located on chromosome 19pter-19p13.3, underscoring genetic significance in human health and disease.

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TIP47 Antibody (B-3) References:

1. Quantitative analysis of TIP47-receptor cytoplasmic domain interactions: implications for endosome-to-trans Golgi network trafficking.  |  Krise, JP., et al. 2000. J Biol Chem. 275: 25188-93. PMID: 10829017
2. Recognition of the 300-kDa mannose 6-phosphate receptor cytoplasmic domain by 47-kDa tail-interacting protein.  |  Orsel, JG., et al. 2000. Proc Natl Acad Sci U S A. 97: 9047-51. PMID: 10908666
3. Overexpression of placental tissue protein 17b/TIP47 in cervical dysplasias and cervical carcinoma.  |  Than, GN., et al. 2001. Anticancer Res. 21: 639-42. PMID: 11299819
4. Self-assembly is important for TIP47 function in mannose 6-phosphate receptor transport.  |  Sincock, PM., et al. 2003. Traffic. 4: 18-25. PMID: 12535272
5. In vitro selection and prediction of TIP47 protein-interaction interfaces.  |  Burguete, AS., et al. 2004. Nat Methods. 1: 55-60. PMID: 15782153
6. Purification and analysis of TIP47 function in Rab9-dependent mannose 6-phosphate receptor trafficking.  |  Burguete, AS., et al. 2005. Methods Enzymol. 403: 357-66. PMID: 16473602
7. Tail-interacting protein TIP47 is a connector between Gag and Env and is required for Env incorporation into HIV-1 virions.  |  Lopez-Vergès, S., et al. 2006. Proc Natl Acad Sci U S A. 103: 14947-52. PMID: 17003132
8. Solution structure studies of monomeric human TIP47/perilipin-3 reveal a highly extended conformation.  |  Hynson, RM., et al. 2012. Proteins. 80: 2046-55. PMID: 22508559
9. Reevaluation of the requirement for TIP47 in human immunodeficiency virus type 1 envelope glycoprotein incorporation.  |  Checkley, MA., et al. 2013. J Virol. 87: 3561-70. PMID: 23325685
10. Epigenetic harnessing of HCV via modulating the lipid droplet-protein, TIP47, in HCV cell models.  |  El-Ekiaby, NM., et al. 2015. FEBS Lett. 589: 2266-73. PMID: 26170028
11. TIP47: a cargo selection device for mannose 6-phosphate receptor trafficking.  |  Díaz, E. and Pfeffer, SR. 1998. Cell. 93: 433-43. PMID: 9590177
12. Cloning and sequence analysis of cDNAs encoding human placental tissue protein 17 (PP17) variants.  |  Than, NG., et al. 1998. Eur J Biochem. 258: 752-7. PMID: 9874244

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TIP47 Antibody (B-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-TIP47 Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TIP47 Antibody (B-3) Specifications

Alternate Product Name: TIP47

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 73-225

Epitope Target: TIP47

Epitope Species: human

Product Reactivity/Detection: TIP47

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for TIP47 Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin