Tim50 Antibody (C-9): sc-393678
- Tim50 Antibody (C-9) is a mouse monoclonal IgG2a κ, cited in 13 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of Tim50 of human origin
- Tim50 Antibody (C-9) is recommended for detection of Tim50 of human origin by WB, IP, IF and ELISA
- Anti-Tim50 Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Tim50 Antibody (C-9) for WB applications. These reagents are now offered in bundles with Tim50 Antibody (C-9) (see ordering information below).
Tim50 Antibody (C-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects Tim50 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tim50 (C-9) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tim50, also known as Tim50L or TIMM50, plays a crucial role in mitochondrial function as a key component of the Tim23 complex, which is essential for importing nuclear-encoded precursor proteins into the mitochondria. Tim50 is primarily located in the inner mitochondrial membrane, where Tim50 facilitates the translocation of proteins necessary for mitochondrial biogenesis and function. Proper functioning of Tim50 is vital, as defects in Tim proteins have been linked to various neurodegenerative diseases, highlighting their importance in cellular health and development. Tim50 exists in two isoforms produced by alternative splicing, with isoform 1 localized to the inner mitochondrial membrane and isoform 2 found in nuclear speckles, indicating a diverse functional repertoire that may influence cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Tim50 Antibody (C-9) References:
- The human family of Deafness/Dystonia peptide (DDP) related mitochondrial import proteins. | Jin, H., et al. 1999. Genomics. 61: 259-67. PMID: 10552927
- The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom. | Bauer, MF., et al. 1999. FEBS Lett. 464: 41-7. PMID: 10611480
- Import of proteins into mitochondria: a novel pathomechanism for progressive neurodegeneration. | Bauer, MF. and Neupert, W. 2001. J Inherit Metab Dis. 24: 166-80. PMID: 11405338
- Tim50, a novel component of the TIM23 preprotein translocase of mitochondria. | Mokranjac, D., et al. 2003. EMBO J. 22: 816-25. PMID: 12574118
- Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death. | Guo, Y., et al. 2004. J Biol Chem. 279: 24813-25. PMID: 15044455
- Tim50a, a nuclear isoform of the mitochondrial Tim50, interacts with proteins involved in snRNP biogenesis. | Xu, H., et al. 2005. BMC Cell Biol. 6: 29. PMID: 16008839
- Tim50 maintains the permeability barrier of the mitochondrial inner membrane. | Meinecke, M., et al. 2006. Science. 312: 1523-6. PMID: 16763150
- Involvement of the mitochondrial protein translocator component tim50 in growth, cell proliferation and the modulation of respiration in Drosophila. | Sugiyama, S., et al. 2007. Genetics. 176: 927-36. PMID: 17435247
- The preprotein translocase of the mitochondrial inner membrane: function and evolution. | Rassow, J., et al. 1999. J Mol Biol. 286: 105-20. PMID: 9931253
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tim50 Antibody (C-9) | sc-393678 | 200 µg/ml | $322.00 | |||
Tim50 Antibody (C-9): m-IgG Fc BP-HRP Bundle | sc-530535 | 200 µg Ab; 10 µg BP | $361.00 | |||
Tim50 Antibody (C-9): m-IgGκ BP-HRP Bundle | sc-524041 | 200 µg Ab, 40 µg BP | $361.00 | |||
Tim50 Antibody (C-9): m-IgG2a BP-HRP Bundle | sc-547519 | 200 µg Ab; 10 µg BP | $361.00 | |||
Tim50 Antibody (C-9) AC | sc-393678 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Tim50 Antibody (C-9) HRP | sc-393678 HRP | 200 µg/ml | $322.00 | |||
Tim50 Antibody (C-9) FITC | sc-393678 FITC | 200 µg/ml | $336.00 | |||
Tim50 Antibody (C-9) PE | sc-393678 PE | 200 µg/ml | $349.00 | |||
Tim50 Antibody (C-9) Alexa Fluor® 488 | sc-393678 AF488 | 200 µg/ml | $364.00 | |||
Tim50 Antibody (C-9) Alexa Fluor® 546 | sc-393678 AF546 | 200 µg/ml | $364.00 | |||
Tim50 Antibody (C-9) Alexa Fluor® 594 | sc-393678 AF594 | 200 µg/ml | $364.00 | |||
Tim50 Antibody (C-9) Alexa Fluor® 647 | sc-393678 AF647 | 200 µg/ml | $364.00 | |||
Tim50 Antibody (C-9) Alexa Fluor® 680 | sc-393678 AF680 | 200 µg/ml | $364.00 | |||
Tim50 Antibody (C-9) Alexa Fluor® 790 | sc-393678 AF790 | 200 µg/ml | $364.00 |