Tim50 Antibody (D-8): sc-515268
- Tim50 Antibody (D-8) is a mouse monoclonal IgG1 κ Tim50 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 203-217 within an internal region of Tim50 of human origin
- Tim50 Antibody (D-8) is recommended for detection of Tim50 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Tim50 Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for Tim50 Antibody (D-8) for WB applications. This reagent is now offered in a bundle with Tim50 Antibody (D-8) (see ordering information below).
Tim50 Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Tim50 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tim50 (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tim50, also known as Tim50L or TIMM50, plays a crucial role in mitochondrial function as a key component of the Tim23 complex, which is essential for importing nuclear-encoded precursor proteins into mitochondria. Tim50 protein is primarily located in the inner mitochondrial membrane, where Tim50 facilitates the translocation of proteins necessary for mitochondrial biogenesis and function. Tim50 function is vital, as defects in Tim50 activity have been linked to various neurodegenerative diseases, highlighting Tim50′s importance in cellular health and development. Two isoforms of Tim50 arise from alternative splicing, with isoform 1 being localized to the inner mitochondrial membrane and isoform 2 found in nuclear speckles, suggesting diverse regulatory roles within the cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tim50 Antibody (D-8) | sc-515268 | 200 µg/ml | $316.00 | |||
Tim50 Antibody (D-8): m-IgG1 BP-HRP Bundle | sc-531804 | 200 µg Ab; 20 µg BP | $354.00 | |||
Tim50 Antibody (D-8) AC | sc-515268 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Tim50 Antibody (D-8) HRP | sc-515268 HRP | 200 µg/ml | $316.00 | |||
Tim50 Antibody (D-8) FITC | sc-515268 FITC | 200 µg/ml | $330.00 | |||
Tim50 Antibody (D-8) PE | sc-515268 PE | 200 µg/ml | $343.00 | |||
Tim50 Antibody (D-8) Alexa Fluor® 488 | sc-515268 AF488 | 200 µg/ml | $357.00 | |||
Tim50 Antibody (D-8) Alexa Fluor® 546 | sc-515268 AF546 | 200 µg/ml | $357.00 | |||
Tim50 Antibody (D-8) Alexa Fluor® 594 | sc-515268 AF594 | 200 µg/ml | $357.00 | |||
Tim50 Antibody (D-8) Alexa Fluor® 647 | sc-515268 AF647 | 200 µg/ml | $357.00 | |||
Tim50 Antibody (D-8) Alexa Fluor® 680 | sc-515268 AF680 | 200 µg/ml | $357.00 | |||
Tim50 Antibody (D-8) Alexa Fluor® 790 | sc-515268 AF790 | 200 µg/ml | $357.00 |