Tim10/TIMM10 Antibody (H-4): sc-518253

Tim10 Antibody (H-4) is a mouse monoclonal IgM antibody specific for an epitope mapping between amino acids 52-74 of Tim10 of human origin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tim10 plays a crucial role in mitochondrial function, specifically in the import of nuclear-encoded precursor proteins into the mitochondria, which is essential for maintaining cellular energy production and overall cellular health. Tim10 protein is part of the mitochondrial Tim protein family, which is vital for the translocation of proteins across the mitochondrial inner membrane. Tim10 forms heteromeric complexes with other Tim proteins, such as Tim9 and Tim12, facilitating the import process by interacting with the carboxy-terminal domain of Tim23, a key component in the mitochondrial import machinery. The proper functioning of Tim proteins, including Tim10, is critical, as defects in these proteins have been linked to various neurodegenerative diseases, highlighting their importance in both development and health. Anti-Tim10 antibody (H-4) is an invaluable tool for researchers studying mitochondrial dynamics and the implications of Tim protein dysfunction in disease.

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Tim10/TIMM10 Antibody (H-4) References:

1. Juxtaposition of the two distal CX3C motifs via intrachain disulfide bonding is essential for the folding of Tim10.  |  Allen, S., et al. 2003. J Biol Chem. 278: 38505-13. PMID: 12882976
2. Reconstituted TOM core complex and Tim9/Tim10 complex of mitochondria are sufficient for translocation of the ADP/ATP carrier across membranes.  |  Vasiljev, A., et al. 2004. Mol Biol Cell. 15: 1445-58. PMID: 14668492
3. The structural basis of the TIM10 chaperone assembly.  |  Lu, H., et al. 2004. J Biol Chem. 279: 18959-66. PMID: 14973126
4. Biogenesis of the essential Tim9-Tim10 chaperone complex of mitochondria: site-specific recognition of cysteine residues by the intermembrane space receptor Mia40.  |  Milenkovic, D., et al. 2007. J Biol Chem. 282: 22472-80. PMID: 17553782
5. Mutation of conserved charged residues in mitochondrial TIM10 subunits precludes TIM10 complex assembly, but does not abolish growth of yeast cells.  |  Vergnolle, MA., et al. 2007. J Mol Biol. 371: 1315-24. PMID: 17618651
6. Zinc binding of Tim10: evidence for existence of an unstructured binding intermediate for a zinc finger protein.  |  Ivanova, E., et al. 2008. Proteins. 71: 467-75. PMID: 17963238
7. Assembly of the mitochondrial Tim9-Tim10 complex: a multi-step reaction with novel intermediates.  |  Ivanova, E., et al. 2008. J Mol Biol. 375: 229-39. PMID: 18022191
8. Identification of the signal directing Tim9 and Tim10 into the intermembrane space of mitochondria.  |  Milenkovic, D., et al. 2009. Mol Biol Cell. 20: 2530-9. PMID: 19297525
9. Temperature-dependent study reveals that dynamics of hydrophobic residues plays an important functional role in the mitochondrial Tim9-Tim10 complex.  |  Ivanova, E., et al. 2012. Proteins. 80: 602-15. PMID: 22095685
10. Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5.  |  Sirrenberg, C., et al. 1998. Nature. 391: 912-5. PMID: 9495346

The preferred Tim10/TIMM10 antibody is Tim10/TIMM10 Antibody (C-7): sc-518241.