Tim10/TIMM10 Antibody (C-7): sc-518241

Tim10 Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody specific for an epitope mapping between amino acids 52-71 of Tim10 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tim10 monoclonal antibody (C-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tim10 plays a crucial role in the import of nuclear-encoded precursor proteins into mitochondria, specifically through involvement in Tim protein complexes that facilitate protein translocation across mitochondrial inner membrane. Tim protein family is essential for mitochondrial function and remains highly conserved across eukaryotic species. Defects in these proteins have been linked to various neurodegenerative diseases, highlighting their importance in cellular health and development. Tim10, located on human chromosome 11q12.1-q12.3, forms heteromeric complexes with Tim9 and Tim12, with Tim9-Tim10 complex playing a significant role in Tim23 import, a key protein in mitochondrial biogenesis. Tim10 and Tim23 interaction is critical, as Tim23′s carboxy-terminal domain contains necessary targeting signals for import, underscoring Tim10′s importance in mitochondrial dynamics and cellular energy metabolism. These features highlight anti-Tim10 antibody (C-7) value as a resource for studying mitochondrial protein import and implications in cellular energy regulation and neurodegenerative diseases.

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Tim10/TIMM10 Antibody (C-7) References:

1. The human family of Deafness/Dystonia peptide (DDP) related mitochondrial import proteins.  |  Jin, H., et al. 1999. Genomics. 61: 259-67. PMID: 10552927
2. The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom.  |  Bauer, MF., et al. 1999. FEBS Lett. 464: 41-7. PMID: 10611480
3. Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria.  |  Davis, AJ., et al. 2000. J Cell Biol. 150: 1271-82. PMID: 10995434
4. Import of proteins into mitochondria: a novel pathomechanism for progressive neurodegeneration.  |  Bauer, MF. and Neupert, W. 2001. J Inherit Metab Dis. 24: 166-80. PMID: 11405338
5. Functional reconstitution of the import of the yeast ADP/ATP carrier mediated by the TIM10 complex.  |  Luciano, P., et al. 2001. EMBO J. 20: 4099-106. PMID: 11483513
6. Zinc binding stabilizes mitochondrial Tim10 in a reduced and import-competent state kinetically.  |  Lu, H. and Woodburn, J. 2005. J Mol Biol. 353: 897-910. PMID: 16199054
7. Assembly of the mitochondrial Tim9-Tim10 complex: a multi-step reaction with novel intermediates.  |  Ivanova, E., et al. 2008. J Mol Biol. 375: 229-39. PMID: 18022191
8. Allosteric and electrostatic protein-protein interactions regulate the assembly of the heterohexameric Tim9-Tim10 complex.  |  Ivanova, E. and Lu, H. 2008. J Mol Biol. 379: 609-16. PMID: 18462749
9. Structural and functional requirements for activity of the Tim9-Tim10 complex in mitochondrial protein import.  |  Baker, MJ., et al. 2009. Mol Biol Cell. 20: 769-79. PMID: 19037098
10. Mitochondrial Tim9 protects Tim10 from degradation by the protease Yme1.  |  Spiller, MP., et al. 2015. Biosci Rep. 35: PMID: 26182355
11. Tim9, a new component of the TIM22.54 translocase in mitochondria.  |  Adam, A., et al. 1999. EMBO J. 18: 313-9. PMID: 9889188
12. The preprotein translocase of the mitochondrial inner membrane: function and evolution.  |  Rassow, J., et al. 1999. J Mol Biol. 286: 105-20. PMID: 9931253

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tim10/TIMM10 Antibody (C-7) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Tim10/TIMM10 Antibody (C-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tim10/TIMM10 Antibody (C-7) Specifications

Alternate Product Name: Tim10

Product Clone Name: C-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 52-71

Epitope Target: Tim10

Epitope Species: human

Product Reactivity/Detection: Tim10

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Tim10/TIMM10 Antibody (C-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin