Tie-2 Antibody (F-8): sc-518137

Tie-2 Antibody (F-8) is a mouse monoclonal IgM antibody that detects Tie-2 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tie-2, also known as Tek, is a receptor tyrosine kinase that plays a crucial role in angiogenesis and vascular stability, making Tie-2 essential for proper blood vessel formation and maintenance. Tie-2 is primarily located on the surface of endothelial cells, where Tie-2 mediates signaling pathways that regulate endothelial cell survival, proliferation, and migration in response to various growth factors. Tie-2′s location in the cell is critical, as activation by ligands such as angiopoietin-1 leads to blood vessel stabilization and prevention of vascular leakage, which is vital for maintaining tissue homeostasis and preventing diseases such as cancer and cardiovascular disorders. Anti-Tie-2 antibody (F-8) is an invaluable tool for researchers studying vascular biology and related pathologies, providing insights into endothelial function mechanisms and therapeutic potential of targeting this receptor in various diseases.

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Tie-2 Antibody (F-8) References:

1. Immunohistochemical localisation of protein tyrosine kinase receptors Tie-1 and Tie-2 in synovial tissue of rheumatoid arthritis: correlation with angiogenesis and synovial proliferation.  |  Uchida, T., et al. 2000. Ann Rheum Dis. 59: 607-14. PMID: 10913057
2. Expression of vascular endothelial growth factor receptor-2 or Tie-2 on peripheral blood cells defines functionally competent cell populations capable of reendothelialization.  |  Nowak, G., et al. 2004. Circulation. 110: 3699-707. PMID: 15381639
3. Placental expression of angiopoietin-1, angiopoietin-2 and tie-2 during placental development in an ovine model of placental insufficiency-fetal growth restriction.  |  Hagen, AS., et al. 2005. Pediatr Res. 58: 1228-32. PMID: 16306198
4. VE-PTP controls blood vessel development by balancing Tie-2 activity.  |  Winderlich, M., et al. 2009. J Cell Biol. 185: 657-71. PMID: 19451274
5. An autocrine linkage between matrix metalloproteinase-14 and Tie-2 via ectodomain shedding modulates angiopoietin-1-dependent function in endothelial cells.  |  Onimaru, M., et al. 2010. Arterioscler Thromb Vasc Biol. 30: 818-26. PMID: 20056911
6. Minimally invasive colorectal resection for cancer is associated with a short-lived decrease in soluble Tie-2 receptor levels, which may transiently inhibit VEGF-mediated angiogenesis (via altered blood levels of free Ang-1 and Ang-2).  |  Shantha Kumara, HM., et al. 2010. Surg Endosc. 24: 2581-7. PMID: 20354881
7. A clinicopathological correlation of the expression of the angiopoietin-Tie-2 receptor pathway in the brain of adults with Plasmodium falciparum malaria.  |  Prapansilp, P., et al. 2013. Malar J. 12: 50. PMID: 23383853
8. Regulation of angiopoietin-1/Tie-2 receptor signaling in endothelial cells by dual-specificity phosphatases 1, 4, and 5.  |  Echavarria, R. and Hussain, SN. 2013. J Am Heart Assoc. 2: e000571. PMID: 24308939
9. [Expression changes of Ang-1, Ang-2, Tie-2 and VEGF in co-culturing of endothelial cells and astrocytes after irradiation by X-ray and their significances].  |  Wu, M., et al. 2014. Zhonghua Yi Xue Za Zhi. 94: 1815-9. PMID: 25154850
10. Gamma-Tocotrienol Induces Apoptosis in Prostate Cancer Cells by Targeting the Ang-1/Tie-2 Signalling Pathway.  |  Tang, KD., et al. 2019. Int J Mol Sci. 20: PMID: 30866453

The preferred Tie-2 antibody is Tie-2 Antibody (F-6): sc-518076.