TGase6 Antibody (E-1): sc-398160
- TGase6 Antibody (E-1) is a mouse monoclonal IgG1 κ TGase6 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 163-188 within an internal region of TGase6 of human origin
- TGase6 Antibody (E-1) is recommended for detection of TGase6 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
- Anti-TGase6 Antibody (E-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TGase6 Antibody (E-1) for WB applications. These reagents are now offered in bundles with TGase6 Antibody (E-1) (see ordering information below).
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TGase6 Antibody (E-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects TGase6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-TGase6 antibody (E-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TGase6, also known as transglutaminase 6, plays a crucial role in forming the cross-linked cell envelope (CE) in terminally differentiating mammalian epidermal cells, which is essential for maintaining skin integrity and barrier function. This 706 amino acid protein catalyzes the cross-linking of proteins and conjugation of proteins to polyamines, facilitating the formation of disulfide bonds and gamma-glutamyl-lysine isodipeptide bonds that contribute to the structural stability of the epidermis. TGase6 is vital for various biological processes, including blood coagulation, epidermal differentiation, and apoptosis, highlighting its importance in both skin health and overall physiological functions. Additionally, TGase6 exists in two isoforms due to alternative splicing, which may have distinct roles in cellular processes, further underscoring the complexity and significance of this protein in biological systems.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TGase6 Antibody (E-1) References:
- Evolution of transglutaminase genes: identification of a transglutaminase gene cluster on human chromosome 15q15. Structure of the gene encoding transglutaminase X and a novel gene family member, transglutaminase Z. | Grenard, P., et al. 2001. J Biol Chem. 276: 33066-78. PMID: 11390390
- Isolation and characterization of cDNA clones to mouse macrophage and human endothelial cell tissue transglutaminases. | Gentile, V., et al. 1991. J Biol Chem. 266: 478-83. PMID: 1670766
- Molecular cloning of human epidermal transglutaminase cDNA from keratinocytes in culture. | Yamanishi, K., et al. 1991. Biochem Biophys Res Commun. 175: 906-13. PMID: 1673840
- Human-immunodeficiency-virus transmembrane glycoprotein gp41 is an amino acceptor and donor substrate for transglutaminase in vitro. | Mariniello, L., et al. 1993. Eur J Biochem. 215: 99-104. PMID: 7688299
- Site-directed mutation in conserved anionic regions of guinea pig liver transglutaminase. | Ikura, K., et al. 1995. Arch Biochem Biophys. 318: 307-13. PMID: 7733658
- Dual functions of transglutaminase in novel cell adhesion. | Ueki, S., et al. 1996. J Cell Sci. 109 (Pt 11): 2727-35. PMID: 8937990
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TGase6 Antibody (E-1) | sc-398160 | 200 µg/ml | $322.00 | |||
TGase6 Antibody (E-1): m-IgG Fc BP-HRP Bundle | sc-530709 | 200 µg Ab; 10 µg BP | $361.00 | |||
TGase6 Antibody (E-1): m-IgGκ BP-HRP Bundle | sc-524237 | 200 µg Ab, 40 µg BP | $361.00 | |||
TGase6 Antibody (E-1): m-IgG1 BP-HRP Bundle | sc-544211 | 200 µg Ab; 20 µg BP | $361.00 | |||
TGase6 Antibody (E-1) AC | sc-398160 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TGase6 Antibody (E-1) HRP | sc-398160 HRP | 200 µg/ml | $322.00 | |||
TGase6 Antibody (E-1) FITC | sc-398160 FITC | 200 µg/ml | $336.00 | |||
TGase6 Antibody (E-1) PE | sc-398160 PE | 200 µg/ml | $349.00 | |||
TGase6 Antibody (E-1) Alexa Fluor® 488 | sc-398160 AF488 | 200 µg/ml | $364.00 | |||
TGase6 Antibody (E-1) Alexa Fluor® 546 | sc-398160 AF546 | 200 µg/ml | $364.00 | |||
TGase6 Antibody (E-1) Alexa Fluor® 594 | sc-398160 AF594 | 200 µg/ml | $364.00 | |||
TGase6 Antibody (E-1) Alexa Fluor® 647 | sc-398160 AF647 | 200 µg/ml | $364.00 | |||
TGase6 Antibody (E-1) Alexa Fluor® 680 | sc-398160 AF680 | 200 µg/ml | $364.00 | |||
TGase6 Antibody (E-1) Alexa Fluor® 790 | sc-398160 AF790 | 200 µg/ml | $364.00 | |||
TGase6 (E-1) Neutralizing Peptide | sc-398160 P | 100 µg/0.5 ml | $69.00 |