TGase2 Antibody (E-3): sc-48387

TGase2 Antibody (E-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects TGase2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). TGase2 (E-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TGase2, also known as transglutaminase 2, plays a crucial role in cellular processes by catalyzing the formation of isopeptide bonds, which are essential for protein cross-linking. This enzymatic activity is particularly important in forming the cross-linked cell envelope (CE) in terminally differentiating mammalian epidermal cells, contributing to structural integrity and barrier function of the epidermis. The CE forms through transglutaminases creating disulfide bonds and γ-glutamyl-lysine isodipeptide bonds, enhancing skin resilience against environmental stressors. TGase2 is involved in blood coagulation, cell differentiation, and apoptosis, highlighting significance in both normal physiology and pathological conditions. Human tissue transglutaminase (TGase2) is a 687 amino acid protein specifically expressed in endothelial cells, making TGase2 a vital target for vascular biology and disease studies.

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TGase2 Antibody (E-3) References:

1. A novel function for transglutaminase 1: attachment of long-chain omega-hydroxyceramides to involucrin by ester bond formation.  |  Nemes, Z., et al. 1999. Proc Natl Acad Sci U S A. 96: 8402-7. PMID: 10411887
2. Regulation of transglutaminases by nitric oxide.  |  Bernassola, F., et al. 1999. Ann N Y Acad Sci. 887: 83-91. PMID: 10668466
3. GTP, an inhibitor of transglutaminases, is hydrolyzed by tissue-type transglutaminase (TGase 2) but not by epidermal-type transglutaminase (TGase 3).  |  Hitomi, K., et al. 2000. Biosci Biotechnol Biochem. 64: 657-9. PMID: 10803976
4. Structure and organization of the human transglutaminase 1 gene.  |  Kim, IG., et al. 1992. J Biol Chem. 267: 7710-7. PMID: 1348508
5. Isolation and characterization of cDNA clones to mouse macrophage and human endothelial cell tissue transglutaminases.  |  Gentile, V., et al. 1991. J Biol Chem. 266: 478-83. PMID: 1670766
6. Molecular cloning of human epidermal transglutaminase cDNA from keratinocytes in culture.  |  Yamanishi, K., et al. 1991. Biochem Biophys Res Commun. 175: 906-13. PMID: 1673840
7. Transglutaminase 2 in cancer.  |  Huang, L., et al. 2015. Am J Cancer Res. 5: 2756-76. PMID: 26609482
8. Transglutaminase 2 in human diseases.  |  Szondy, Z., et al. 2017. Biomedicine (Taipei). 7: 15. PMID: 28840829
9. Transglutaminase 2 mediates transcriptional regulation through BAF250a polyamination.  |  Kim, HJ., et al. 2021. Genes Genomics. 43: 333-342. PMID: 33555506
10. Transglutaminase 2 inhibitor for coeliac disease.  |  Ray, K. 2021. Nat Rev Gastroenterol Hepatol. 18: 594. PMID: 34267378
11. Silencing Transglutaminase Genes TGase2 and TGase3 Has Infection-Dependent Effects on the Heart Rate of the Mosquito Anopheles gambiae.  |  Ramakrishnan, A. and Hillyer, JF. 2022. Insects. 13: PMID: 35886758
12. Dual functions of transglutaminase in novel cell adhesion.  |  Ueki, S., et al. 1996. J Cell Sci. 109 (Pt 11): 2727-35. PMID: 8937990

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TGase2 Antibody (E-3) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-TGase2 Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TGase2 Antibody (E-3) Specifications

Alternate Product Name: TGase2

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 451-687

Epitope Target: TGase2

Epitope Species: human

Product Reactivity/Detection: TGase2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for TGase2 Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin