TdT Antibody (C-11): sc-393710
- TdT Antibody (C-11) is a mouse monoclonal IgG2b κ TdT antibody provided at 200 µg/ml
- raised against amino acids 450-509 mapping at the C-terminus of TdT of human origin
- TdT Antibody (C-11) is recommended for detection of TdT of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TdT Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TdT Antibody (C-11) for WB applications. These reagents are now offered in bundles with TdT Antibody (C-11) (see ordering information below).
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TdT Antibody (C-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects TdT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-TdT antibody (C-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Terminal deoxynucleotidyltransferase (TdT) plays a crucial role in DNA synthesis by catalyzing the addition of deoxyribonucleotides to the 3′-hydroxyl end of DNA primers without the need for a template, which is essential for precise labeling of DNA′s 3′ termini. The human TdT gene is located on chromosome 10q23-q24 and encodes a 510 amino acid protein that is synthesized as a single-chain peptide. This enzyme exhibits a slight preference for incorporating deoxyribonucleotides over ribonucleotides, which is significant for maintaining DNA strand integrity during replication and repair processes. TdT is predominantly found in immature thymocytes, certain bone marrow cells, transformed pre-B and pre-T cell lines, as well as in leukemia cells, highlighting its importance in both normal and pathological cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TdT Antibody (C-11) References:
- Methylation of the promoter region may be involved in tissue-specific expression of the mouse terminal deoxynucleotidyl transferase gene. | Nourrit, F., et al. 1999. J Mol Biol. 292: 217-27. PMID: 10493870
- Forced expression of terminal deoxynucleotidyl transferase in fetal thymus resulted in a decrease in gammadelta T cells and random dissemination of Vgamma3Vdelta1 T cells in skin of newborn but not adult mice. | Aono, A., et al. 2000. Immunology. 99: 489-97. PMID: 10792495
- Terminal deoxynucleotidyl transferase indiscriminately incorporates ribonucleotides and deoxyribonucleotides. | Boulé, JB., et al. 2001. J Biol Chem. 276: 31388-93. PMID: 11406636
- Terminal deoxynucleotidyl transferase deficiency decreases autoimmune disease in MRL-Fas(lpr) mice. | Feeney, AJ., et al. 2001. J Immunol. 167: 3486-93. PMID: 11544342
- Role of human Pso4 in mammalian DNA repair and association with terminal deoxynucleotidyl transferase. | Mahajan, KN. and Mitchell, BS. 2003. Proc Natl Acad Sci U S A. 100: 10746-51. PMID: 12960389
- Terminal deoxynucleotidyl transferase is down-regulated by AP-1-like regulatory elements in human lymphoid cells. | Peralta-Zaragoza, O., et al. 2004. Immunology. 111: 195-203. PMID: 15027905
- Constitutive expression of terminal deoxynucleotidyl transferase in transgenic mice is sufficient for N region diversity to occur at any Ig locus throughout B cell differentiation. | Bentolila, LA., et al. 1997. J Immunol. 158: 715-23. PMID: 8992987
- Terminal deoxynucleotidyl transferase expression during neonatal life alters D(H) reading frame usage and Ig-receptor-dependent selection of V regions. | Marshall, AJ., et al. 1998. J Immunol. 161: 6657-63. PMID: 9862694
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TdT Antibody (C-11) | sc-393710 | 200 µg/ml | $316.00 | |||
TdT Antibody (C-11): m-IgG Fc BP-HRP Bundle | sc-530551 | 200 µg Ab; 10 µg BP | $354.00 | |||
TdT Antibody (C-11): m-IgGκ BP-HRP Bundle | sc-524057 | 200 µg Ab, 40 µg BP | $354.00 | |||
TdT Antibody (C-11): m-IgG2b BP-HRP Bundle | sc-550199 | 200 µg Ab; 10 µg BP | $354.00 | |||
TdT Antibody (C-11) AC | sc-393710 AC | 500 µg/ml, 25% agarose | $416.00 | |||
TdT Antibody (C-11) HRP | sc-393710 HRP | 200 µg/ml | $316.00 | |||
TdT Antibody (C-11) FITC | sc-393710 FITC | 200 µg/ml | $330.00 | |||
TdT Antibody (C-11) PE | sc-393710 PE | 200 µg/ml | $343.00 | |||
TdT Antibody (C-11) Alexa Fluor® 488 | sc-393710 AF488 | 200 µg/ml | $357.00 | |||
TdT Antibody (C-11) Alexa Fluor® 546 | sc-393710 AF546 | 200 µg/ml | $357.00 | |||
TdT Antibody (C-11) Alexa Fluor® 594 | sc-393710 AF594 | 200 µg/ml | $357.00 | |||
TdT Antibody (C-11) Alexa Fluor® 647 | sc-393710 AF647 | 200 µg/ml | $357.00 | |||
TdT Antibody (C-11) Alexa Fluor® 680 | sc-393710 AF680 | 200 µg/ml | $357.00 | |||
TdT Antibody (C-11) Alexa Fluor® 790 | sc-393710 AF790 | 200 µg/ml | $357.00 |