TdT Antibody (B-9): sc-398238

TdT Antibody (B-9) is a mouse monoclonal IgG2a light chain antibody that detects Terminal deoxynucleotidyltransferase (TdT) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TdT is a crucial DNA polymerase that catalyzes the addition of deoxyribonucleotides to the 3′-hydroxyl end of DNA primers without the need for a template, which is essential for the unique labeling of the 3′ termini of DNA. This enzyme plays a significant role in DNA synthesis and repair, particularly during the development of the immune system, where TdT is expressed in immature thymocytes and certain leukemia cells. The human TdT gene is located on chromosome 10q23-q24 and encodes a 510 amino acid protein that exhibits a preference for incorporating deoxyribonucleotides over ribonucleotides, thereby forming DNA strands. TdT expression in various cell types, including transformed pre-B and pre-T cell lines, highlights anti-TdT antibody (B-9)′s importance in both normal and pathological processes, making this tool invaluable for researchers studying DNA metabolism and cellular differentiation.

The preferred TdT antibody is TdT Antibody (C-11): sc-393710.