TBK1 Antibody (A-6): sc-398366

TBK1 Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects TBK1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-TBK1 antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TBK1 plays a crucial role in regulating immune response and inflammation, particularly through involvement in activating transcription factor NF-kB. This activation is significant because NF-kB drives expression of various pro-inflammatory cytokines and survival factors, which are vital for immune system response to pathogens and stress. TBK1 interacts with other proteins in NF-kB signaling pathway, including TRAF2 and TANK, facilitating phosphorylation and degradation of inhibitory protein IkB, which allows NF-kB to translocate to nucleus and activate target genes. TBK1′s ability to modulate this pathway underscores its importance in both innate immunity and pathogenesis of various inflammatory diseases, making TBK1 (A-6) monoclonal antibody a valuable tool for researchers studying these processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TBK1 Antibody (A-6) References:

1. STING specifies IRF3 phosphorylation by TBK1 in the cytosolic DNA signaling pathway.  |  Tanaka, Y. and Chen, ZJ. 2012. Sci Signal. 5: ra20. PMID: 22394562
2. TRIM23 mediates virus-induced autophagy via activation of TBK1.  |  Sparrer, KMJ., et al. 2017. Nat Microbiol. 2: 1543-1557. PMID: 28871090
3. Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during Selective Autophagy.  |  Vargas, JNS., et al. 2019. Mol Cell. 74: 347-362.e6. PMID: 30853401
4. TBK1 recruitment to STING activates both IRF3 and NF-κB that mediate immune defense against tumors and viral infections.  |  Yum, S., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33785602
5. Tiliroside targets TBK1 to induce ferroptosis and sensitize hepatocellular carcinoma to sorafenib.  |  Yang, C., et al. 2023. Phytomedicine. 111: 154668. PMID: 36657316
6. VANGL2 inhibits antiviral IFN-I signaling by targeting TBK1 for autophagic degradation.  |  Hu, Z., et al. 2023. Sci Adv. 9: eadg2339. PMID: 37352355
7. ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1.  |  Xie, W., et al. 2023. Autophagy. 19: 2853-2868. PMID: 37434364
8. Optineurin provides a mitophagy contact site for TBK1 activation.  |  Yamano, K., et al. 2024. EMBO J. 43: 754-779. PMID: 38287189
9. A TBK1 variant causes autophagolysosomal and motoneuron pathology without neuroinflammation in mice.  |  Brenner, D., et al. 2024. J Exp Med. 221: PMID: 38517332
10. CDK4/6 Alters TBK1 Phosphorylation to Inhibit the STING Signaling Pathway in Prostate Cancer.  |  Li, W., et al. 2024. Cancer Res. 84: 2588-2606. PMID: 38861362

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TBK1 Antibody (A-6) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-TBK1 Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TBK1 Antibody (A-6) Specifications

Alternate Product Name: TANK binding kinase 1

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 355-729

Epitope Target: TBK1

Epitope Species: mouse

Product Reactivity/Detection: TBK1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for TBK1 Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred TBK1 antibody is TBK1 Antibody (108A429): sc-52957.