TAZ Antibody (1F1): sc-293183

TAZ Antibody (1F1) is a mouse monoclonal IgG2a kappa light chain antibody that detects TAZ protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-TAZ antibody (1F1) is available as the non-conjugated format. The transcriptional co-activator with PDZ-binding motif (TAZ), also known as WWTR1, plays a crucial role in cellular signaling and gene expression regulation. TAZ is primarily located in the cytoplasm and nucleus, where TAZ acts as a key mediator in the Hippo signaling pathway, which is essential for controlling cell growth, differentiation, and apoptosis. Proper localization of TAZ is vital because nuclear translocation allows TAZ to interact with various transcription factors, thereby influencing gene expression that governs cell proliferation and survival. Dysregulation of TAZ localization can lead to uncontrolled cell growth and has been implicated in various cancers. TAZ (1F1) antibody recognizes immunoreactive protein bands in lysates from MDCK, NIH-3T3, and 293T cells, and TAZ is also expressed in COS7, Hep G2, CHO, and HeLa cells. Notably, TAZ binding to 14-3-3 proteins is contingent upon phosphorylation at Serine 89, which subsequently inhibits transcriptional co-activation through 14-3-3-mediated nuclear export, highlighting the importance of post-translational modifications in regulating TAZ function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TAZ Antibody (1F1) References:

1. 14-3-3 proteins and growth control.  |  Baldin, V. 2000. Prog Cell Cycle Res. 4: 49-60. PMID: 10740814
2. 14-3-3 proteins: structure, function, and regulation.  |  Fu, H., et al. 2000. Annu Rev Pharmacol Toxicol. 40: 617-47. PMID: 10836149
3. PDZ domains in synapse assembly and signalling.  |  Garner, CC., et al. 2000. Trends Cell Biol. 10: 274-80. PMID: 10856930
4. TAZ: a novel transcriptional co-activator regulated by interactions with 14-3-3 and PDZ domain proteins.  |  Kanai, F., et al. 2000. EMBO J. 19: 6778-91. PMID: 11118213
5. 14-3-3 proteins: regulation of subcellular localization by molecular interference.  |  Muslin, AJ. and Xing, H. 2000. Cell Signal. 12: 703-9. PMID: 11152955
6. YAP/TAZ at the Roots of Cancer.  |  Zanconato, F., et al. 2016. Cancer Cell. 29: 783-803. PMID: 27300434
7. YAP/TAZ upstream signals and downstream responses.  |  Totaro, A., et al. 2018. Nat Cell Biol. 20: 888-899. PMID: 30050119
8. Interplay between YAP/TAZ and Metabolism.  |  Koo, JH. and Guan, KL. 2018. Cell Metab. 28: 196-206. PMID: 30089241
9. Hippo-YAP/TAZ signalling in organ regeneration and regenerative medicine.  |  Moya, IM. and Halder, G. 2019. Nat Rev Mol Cell Biol. 20: 211-226. PMID: 30546055
10. YAP/TAZ Signaling and Resistance to Cancer Therapy.  |  Nguyen, CDK. and Yi, C. 2019. Trends Cancer. 5: 283-296. PMID: 31174841
11. YAP/TAZ and ATF4 drive resistance to Sorafenib in hepatocellular carcinoma by preventing ferroptosis.  |  Gao, R., et al. 2021. EMBO Mol Med. 13: e14351. PMID: 34664408
12. YAP/TAZ as master regulators in cancer: modulation, function and therapeutic approaches.  |  Piccolo, S., et al. 2023. Nat Cancer. 4: 9-26. PMID: 36564601

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TAZ Antibody (1F1) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-TAZ Antibody (1F1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TAZ Antibody (1F1) Specifications

Alternate Product Name: TAZ

Product Clone Name: 1F1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 201-300

Epitope Target: TAZ

Epitope Species: human

Product Reactivity/Detection: TAZ

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for TAZ Antibody (1F1): N/A

The preferred TAZ antibody is TAZ Antibody (D-8): sc-518026.