Tau Antibody (E-4): sc-515539

Tau Antibody (E-4) is a mouse monoclonal IgG2b kappa light chain antibody engineered to detect Tau protein, also known as MAPT (microtubule-associated protein tau), or TAU. Anti-Tau antibody (E-4) recognizes an epitope mapping between amino acids 358-379 at the C-terminus of the CNS isoform of human Tau, ensuring effective detection of Tau isoforms from mouse, rat, and human sources through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tau monoclonal antibody (E-4) is available in both non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, providing versatility for a wide range of experimental applications. The Tau protein is a 758 amino acid-long protein predominantly located in the cytoplasm, cytoskeleton, and cell membrane of neuronal cells, where Tau plays a vital role in promoting microtubule assembly and stability. This localization is crucial for maintaining neuronal structure, facilitating intracellular transport, and supporting neuronal polarity, which are essential for proper neuronal function and communication. Tau interacts with key proteins such as kinases like GSK-3β and phosphatases like PP2A, which regulate Tau phosphorylation state and functional activity. Proper functioning of Tau is essential for cytoskeletal integrity and neuronal health, while Tau dysregulation and aggregation are implicated in the development of neurodegenerative diseases. Abnormal Tau accumulation and hyperphosphorylation are hallmark features of Alzheimer′s disease, corticobasal degeneration (CBD), progressive supranuclear palsy (PSP), and other tauopathies, making Tau (E-4) antibody an invaluable tool for researchers studying these conditions.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tau Antibody (E-4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Tau Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tau Antibody (E-4) Specifications

Alternate Product Name: MAPT

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 358-379

Epitope Target: Tau (CNS isoform)

Epitope Species: human

Product Reactivity/Detection: multiple Tau isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Tau Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Tau antibody is Tau Antibody (Tau 46): sc-32274.