TANC Antibody (H-8): sc-514679
- TANC Antibody (H-8) is a mouse monoclonal IgG2a κ TANC antibody provided at 200 µg/ml
- raised against amino acids 629-679 mapping within an internal region of TANC of human origin
- TANC Antibody (H-8) is recommended for detection of TANC of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TANC Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TANC Antibody (H-8) for WB applications. These reagents are now offered in bundles with TANC Antibody (H-8) (see ordering information below).
QUICK LINKS
TANC Antibody (H-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects TANC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-TANC antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TANC protein, also known as TANC1, is a crucial postsynaptic cell membrane protein composed of 1,861 amino acids, featuring eleven ankyrin repeats and three tetratricopeptide repeats, which categorize TANC1 within the TANC family. TANC1′s primary function as a scaffolding component in the postsynaptic density is vital for synaptic signaling and plasticity, as TANC1 facilitates interactions with key proteins such as TNIK, SAPAP1, α-internexin, CaMKII, NMDA receptor subunit ε2, and GluR-1. These interactions are essential for the organization and stability of the postsynaptic structure, influencing synaptic strength and neuronal communication. TANC1 interacts directly with other important scaffolding proteins like SAP 97, PSD-95, and Homer, which further underscores TANC1′s role in maintaining synaptic integrity. Upon stimulation by Rap 2, TANC1 can be phosphorylated by MINK1 and TNIK, adding another layer of regulation to TANC1′s function. The TANC gene encodes two alternatively spliced isoforms, spans approximately 264,025 bases, and is located on human chromosome 2q24.2, a region associated with various genetic disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TANC Antibody (H-8) References:
- Alstrom syndrome: confirmation of linkage to chromosome 2p12-13 and phenotypic heterogeneity in three affected sibs. | Zumsteg, U., et al. 2000. J Med Genet. 37: E8. PMID: 10882760
- An ATP-binding cassette gene (ABCG5) from the ABCG (White) gene subfamily maps to human chromosome 2p21 in the region of the Sitosterolemia locus. | Shulenin, S., et al. 2001. Cytogenet Cell Genet. 92: 204-8. PMID: 11435688
- Mutation of ALMS1, a large gene with a tandem repeat encoding 47 amino acids, causes Alström syndrome. | Hearn, T., et al. 2002. Nat Genet. 31: 79-83. PMID: 11941370
- A novel scaffold protein, TANC, possibly a rat homolog of Drosophila rolling pebbles (rols), forms a multiprotein complex with various postsynaptic density proteins. | Suzuki, T., et al. 2005. Eur J Neurosci. 21: 339-50. PMID: 15673434
- Mutations in ABCA12 underlie the severe congenital skin disease harlequin ichthyosis. | Kelsell, DP., et al. 2005. Am J Hum Genet. 76: 794-803. PMID: 15756637
- MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1. | Nonaka, H., et al. 2008. Biochem Biophys Res Commun. 377: 573-578. PMID: 18930710
- Mapping a gene involved in regulating dietary cholesterol absorption. The sitosterolemia locus is found at chromosome 2p21. | Patel, SB., et al. 1998. J Clin Invest. 102: 1041-4. PMID: 9727073
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TANC Antibody (H-8) | sc-514679 | 200 µg/ml | $322.00 | |||
TANC Antibody (H-8): m-IgG Fc BP-HRP Bundle | sc-531190 | 200 µg Ab; 10 µg BP | $361.00 | |||
TANC Antibody (H-8): m-IgGκ BP-HRP Bundle | sc-524810 | 200 µg Ab, 40 µg BP | $361.00 | |||
TANC Antibody (H-8): m-IgG2a BP-HRP Bundle | sc-547720 | 200 µg Ab; 10 µg BP | $361.00 | |||
TANC Antibody (H-8) AC | sc-514679 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TANC Antibody (H-8) HRP | sc-514679 HRP | 200 µg/ml | $322.00 | |||
TANC Antibody (H-8) FITC | sc-514679 FITC | 200 µg/ml | $336.00 | |||
TANC Antibody (H-8) PE | sc-514679 PE | 200 µg/ml | $349.00 | |||
TANC Antibody (H-8) Alexa Fluor® 488 | sc-514679 AF488 | 200 µg/ml | $364.00 | |||
TANC Antibody (H-8) Alexa Fluor® 546 | sc-514679 AF546 | 200 µg/ml | $364.00 | |||
TANC Antibody (H-8) Alexa Fluor® 594 | sc-514679 AF594 | 200 µg/ml | $364.00 | |||
TANC Antibody (H-8) Alexa Fluor® 647 | sc-514679 AF647 | 200 µg/ml | $364.00 | |||
TANC Antibody (H-8) Alexa Fluor® 680 | sc-514679 AF680 | 200 µg/ml | $364.00 | |||
TANC Antibody (H-8) Alexa Fluor® 790 | sc-514679 AF790 | 200 µg/ml | $364.00 |