TAF II p28 Antibody (A-4): sc-393100
- TAF II p28 Antibody (A-4) is a mouse monoclonal IgG2b κ TAF II p28 antibody provided at 200 µg/ml
- raised against amino acids 54-211 mapping at the C-terminus of TAF II p28 of human origin
- TAF II p28 Antibody (A-4) is recommended for detection of TAF II p28 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TAF II p28 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TAF II p28 Antibody (A-4) for WB applications. These reagents are now offered in bundles with TAF II p28 Antibody (A-4) (see ordering information below).
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TAF II p28 Antibody (A-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects TAF II p28 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TAF II p28 (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TAF II p28 plays a crucial role in the assembly of transcription factor TFIID, which is essential for preinitiation complex formation at the TATA promoter element. TAF II p28 functions as part of a larger multisubunit complex that includes several TBP-associated factors (TAFs). TAF II p28 interacts with other TAFs, such as TAF II p18, to enhance transcriptional activation, particularly in nuclear hormone receptors like estrogen receptor and vitamin D3 receptor. TAF II p28 also participates in retinoid X receptor alpha activation pathway as a limiting factor, underscoring TAF II p28′s importance in gene regulation. TAF II p28′s ability to modulate transcriptional responses highlights TAF II p28′s significance in cellular processes, including differentiation and apoptosis, making TAF II p28 a valuable target for research in gene expression and regulatory mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TAF II p28 Antibody (A-4) References:
- Repression of A TAF(II)32 isoform as part of a program of genes regulated during mpl ligand-induced megakaryocyte differentiation. | Thompson, A. and Ravid, K. 1999. Biochem Biophys Res Commun. 262: 55-9. PMID: 10448067
- Identification of human TFIID components and direct interaction between a 250-kDa polypeptide and the TATA box-binding protein (TFIID tau). | Takada, R., et al. 1992. Proc Natl Acad Sci U S A. 89: 11809-13. PMID: 1465404
- Isolation of coactivators associated with the TATA-binding protein that mediate transcriptional activation. | Dynlacht, BD., et al. 1991. Cell. 66: 563-76. PMID: 1907890
- Five intermediate complexes in transcription initiation by RNA polymerase II. | Buratowski, S., et al. 1989. Cell. 56: 549-61. PMID: 2917366
- Multiple factors required for accurate initiation of transcription by purified RNA polymerase II. | Matsui, T., et al. 1980. J Biol Chem. 255: 11992-6. PMID: 7440580
- Molecular cloning and expression of the 32-kDa subunit of human TFIID reveals interactions with VP16 and TFIIB that mediate transcriptional activation. | Klemm, RD., et al. 1995. Proc Natl Acad Sci U S A. 92: 5788-92. PMID: 7597030
- Cloning and characterization of hTAFII18, hTAFII20 and hTAFII28: three subunits of the human transcription factor TFIID. | Mengus, G., et al. 1995. EMBO J. 14: 1520-31. PMID: 7729427
- Human TAF(II28) promotes transcriptional stimulation by activation function 2 of the retinoid X receptors. | May, M., et al. 1996. EMBO J. 15: 3093-104. PMID: 8670810
- The class II trans-activator CIITA interacts with the TBP-associated factor TAFII32. | Fontes, JD., et al. 1997. Nucleic Acids Res. 25: 2522-8. PMID: 9171108
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAF II p28 Antibody (A-4) | sc-393100 | 200 µg/ml | $322.00 | |||
TAF II p28 Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-530355 | 200 µg Ab; 10 µg BP | $361.00 | |||
TAF II p28 Antibody (A-4): m-IgGκ BP-HRP Bundle | sc-523805 | 200 µg Ab, 40 µg BP | $361.00 | |||
TAF II p28 Antibody (A-4) AC | sc-393100 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TAF II p28 Antibody (A-4) HRP | sc-393100 HRP | 200 µg/ml | $322.00 | |||
TAF II p28 Antibody (A-4) FITC | sc-393100 FITC | 200 µg/ml | $336.00 | |||
TAF II p28 Antibody (A-4) PE | sc-393100 PE | 200 µg/ml | $349.00 | |||
TAF II p28 Antibody (A-4) Alexa Fluor® 488 | sc-393100 AF488 | 200 µg/ml | $364.00 | |||
TAF II p28 Antibody (A-4) Alexa Fluor® 546 | sc-393100 AF546 | 200 µg/ml | $364.00 | |||
TAF II p28 Antibody (A-4) Alexa Fluor® 594 | sc-393100 AF594 | 200 µg/ml | $364.00 | |||
TAF II p28 Antibody (A-4) Alexa Fluor® 647 | sc-393100 AF647 | 200 µg/ml | $364.00 | |||
TAF II p28 Antibody (A-4) Alexa Fluor® 680 | sc-393100 AF680 | 200 µg/ml | $364.00 | |||
TAF II p28 Antibody (A-4) Alexa Fluor® 790 | sc-393100 AF790 | 200 µg/ml | $364.00 |