SUV39H1 Antibody (44.1): sc-23961

SUV39H1 Antibody (44.1) is a mouse monoclonal IgG1 kappa light chain antibody that detects SUV39H1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-SUV39H1 antibody (44.1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SUV39H1 protein plays a crucial role in epigenetic regulation by specifically methylating Lysine 9 on the amino terminus of Histone H3, which creates a binding site for heterochromatin protein 1 (HP1). This interaction is vital for gene silencing and the maintenance of higher-order chromatin structures, influencing chromosome function and overall gene expression. SUV39H1 is characterized by the presence of both SET and chromo domains, and expression is ubiquitous across various tissues. Notably, SUV39H1 localization at heterochromatic foci during interphase and centromere-specific presence during metaphase are dependent on the C-terminal SET domain. Additionally, SUV39H1 undergoes phosphorylation at the G1/S cell cycle transition, which is significant for regulatory functions, as forced expression can suppress cell growth. SUV39H1 monoclonal antibody (44.1) is an essential tool for researchers studying chromatin dynamics, gene regulation, and the intricate mechanisms of epigenetic control.

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SUV39H1 Antibody (44.1) References:

1. Functional mammalian homologues of the Drosophila PEV-modifier Su(var)3-9 encode centromere-associated proteins which complex with the heterochromatin component M31.  |  Aagaard, L., et al. 1999. EMBO J. 18: 1923-38. PMID: 10202156
2. Structure-function analysis of SUV39H1 reveals a dominant role in heterochromatin organization, chromosome segregation, and mitotic progression.  |  Melcher, M., et al. 2000. Mol Cell Biol. 20: 3728-41. PMID: 10779362
3. Set domain-dependent regulation of transcriptional silencing and growth control by SUV39H1, a mammalian ortholog of Drosophila Su(var)3-9.  |  Firestein, R., et al. 2000. Mol Cell Biol. 20: 4900-9. PMID: 10848615
4. Regulation of chromatin structure by site-specific histone H3 methyltransferases.  |  Rea, S., et al. 2000. Nature. 406: 593-9. PMID: 10949293
5. Gene regulation of Wiskott-Aldrich syndrome protein and the human homolog of the Drosophila Su(var)3-9: WASP and SUV39H1, two adjacent genes at Xp11.23.  |  Hagemann, TL., et al. 2000. Biochim Biophys Acta. 1493: 368-72. PMID: 11018264
6. Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins.  |  Lachner, M., et al. 2001. Nature. 410: 116-20. PMID: 11242053
7. Set domain-containing protein, G9a, is a novel lysine-preferring mammalian histone methyltransferase with hyperactivity and specific selectivity to lysines 9 and 27 of histone H3.  |  Tachibana, M., et al. 2001. J Biol Chem. 276: 25309-17. PMID: 11316813
8. SUV39H1 Ablation Enhances Long-term CAR T Function in Solid Tumors.  |  López-Cobo, S., et al. 2024. Cancer Discov. 14: 120-141. PMID: 37934001
9. Disruption of SUV39H1-Mediated H3K9 Methylation Sustains CAR T-cell Function.  |  Jain, N., et al. 2024. Cancer Discov. 14: 142-157. PMID: 37934007
10. Chaetocin-mediated SUV39H1 inhibition targets stemness and oncogenic networks of diffuse midline gliomas and synergizes with ONC201.  |  Xin, DE., et al. 2024. Neuro Oncol. 26: 735-748. PMID: 38011799
11. Histone methyltransferase Suv39h1 regulates hepatic stellate cell activation and is targetable in liver fibrosis.  |  Kong, M., et al. 2024. Gut. 73: 810-824. PMID: 38176898
12. METTL3/METTL14 maintain human nucleoli integrity by mediating SUV39H1/H2 degradation.  |  Shan, Y., et al. 2024. Nat Commun. 15: 7186. PMID: 39169036

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SUV39H1 Antibody (44.1) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-SUV39H1 Antibody (44.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SUV39H1 Antibody (44.1) Specifications

Alternate Product Name: KMT1A

Product Clone Name: 44.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 380-393

Epitope Target: SUV39H1

Epitope Species: human

Product Reactivity/Detection: N-terminal (195 aa) SUV39H1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for SUV39H1 Antibody (44.1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred SUV39H1 antibody is SUV39H1 Antibody (C-10): sc-377112.