SSH3 Antibody (G-2): sc-374560
- SSH3 Antibody (G-2) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 431-659 mapping at the C-terminus of SSH3 of human origin
- SSH3 Antibody (G-2) is recommended for detection of SSH3 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-SSH3 Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SSH3 Antibody (G-2) for WB and IHC(P) applications. These reagents are now offered in bundles with SSH3 Antibody (G-2) (see ordering information below).
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SSH3 Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects SSH3 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). SSH3 (G-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SSH3, also known as Slingshot homolog 3 or SSH3L, is a 659 amino acid protein that plays a crucial role in regulating actin filament dynamics, which is essential for various cellular processes such as motility, shape, and division. SSH3 is localized in both the nucleus and cytoplasm, allowing SSH3 to interact with key regulatory proteins involved in actin remodeling. SSH3 functions as a protein phosphatase, specifically targeting the ADF/Cofilin family of proteins, which are critical for the rapid depolymerization and disassembly of filamentous actin (F-actin) in response to cellular signals. By dephosphorylating ADF and Cofilin, SSH3 reactivates these proteins, enabling them to effectively regulate actin dynamics and maintain cellular architecture. SSH3 is expressed as five isoforms due to alternative splicing, which may contribute to SSH3′s functional diversity and regulatory mechanisms in different cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SSH3 Antibody (G-2) References:
- Proteins of the ADF/cofilin family: essential regulators of actin dynamics. | Bamburg, JR. 1999. Annu Rev Cell Dev Biol. 15: 185-230. PMID: 10611961
- Role of native topology investigated by multiple unfolding simulations of four SH3 domains. | Gsponer, J. and Caflisch, A. 2001. J Mol Biol. 309: 285-98. PMID: 11491296
- Control of actin reorganization by Slingshot, a family of phosphatases that dephosphorylate ADF/cofilin. | Niwa, R., et al. 2002. Cell. 108: 233-46. PMID: 11832213
- Regulation of growth cone actin dynamics by ADF/cofilin. | Gungabissoon, RA. and Bamburg, JR. 2003. J Histochem Cytochem. 51: 411-20. PMID: 12642619
- Differential activities, subcellular distribution and tissue expression patterns of three members of Slingshot family phosphatases that dephosphorylate cofilin. | Ohta, Y., et al. 2003. Genes Cells. 8: 811-24. PMID: 14531860
- Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin. | Soosairajah, J., et al. 2005. EMBO J. 24: 473-86. PMID: 15660133
- The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization, and motility behavior of keratinocytes. | Kligys, K., et al. 2007. J Biol Chem. 282: 32520-8. PMID: 17848544
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SSH3 Antibody (G-2) | sc-374560 | 200 µg/ml | $316.00 | |||
SSH3 Antibody (G-2): m-IgG Fc BP-HRP Bundle | sc-529771 | 200 µg Ab; 10 µg BP | $354.00 | |||
SSH3 Antibody (G-2): m-IgGκ BP-HRP Bundle | sc-522777 | 200 µg Ab, 40 µg BP | $354.00 | |||
SSH3 Antibody (G-2): m-IgG1 BP-HRP Bundle | sc-543681 | 200 µg Ab; 20 µg BP | $354.00 | |||
SSH3 Antibody (G-2) AC | sc-374560 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SSH3 Antibody (G-2) HRP | sc-374560 HRP | 200 µg/ml | $316.00 | |||
SSH3 Antibody (G-2) FITC | sc-374560 FITC | 200 µg/ml | $330.00 | |||
SSH3 Antibody (G-2) PE | sc-374560 PE | 200 µg/ml | $343.00 | |||
SSH3 Antibody (G-2) Alexa Fluor® 488 | sc-374560 AF488 | 200 µg/ml | $357.00 | |||
SSH3 Antibody (G-2) Alexa Fluor® 546 | sc-374560 AF546 | 200 µg/ml | $357.00 | |||
SSH3 Antibody (G-2) Alexa Fluor® 594 | sc-374560 AF594 | 200 µg/ml | $357.00 | |||
SSH3 Antibody (G-2) Alexa Fluor® 647 | sc-374560 AF647 | 200 µg/ml | $357.00 | |||
SSH3 Antibody (G-2) Alexa Fluor® 680 | sc-374560 AF680 | 200 µg/ml | $357.00 | |||
SSH3 Antibody (G-2) Alexa Fluor® 790 | sc-374560 AF790 | 200 µg/ml | $357.00 |