spectrin β IV Antibody (F-5): sc-398506

spectrin β IV Antibody (F-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects spectrin β IV protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-spectrin β IV antibody (F-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Spectrin, a crucial actin-binding protein, plays a vital role in maintaining the structural integrity and functionality of the cytoskeletal framework, particularly in the erythrocyte plasma membrane, where spectrin influences membrane shape and deformability. spectrin β IV monoclonal antibody (F-5) targets a protein predominantly expressed in the brain and pancreatic islets, where spectrin β IV localizes to the nuclear matrix, cytoplasmic vesicles, and PML nuclear bodies. spectrin β IV (F-5) antibody recognizes a protein composed of 2,564 amino acids that exists in four isoforms due to alternative splicing, allowing for functional diversity and adaptability in various cellular contexts. spectrin β IV (F-5) monoclonal antibody enables researchers to study a protein that interacts with other proteins involved in cellular signaling and structural organization, contributing to maintaining cellular architecture and facilitating intracellular transport.

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spectrin β IV Antibody (F-5) References:

1. Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.  |  Nagase, T., et al. 2000. DNA Res. 7: 273-81. PMID: 10997877
2. A new spectrin, beta IV, has a major truncated isoform that associates with promyelocytic leukemia protein nuclear bodies and the nuclear matrix.  |  Tse, WT., et al. 2001. J Biol Chem. 276: 23974-85. PMID: 11294830
3. Erythroid expression of the human alpha-spectrin gene promoter is mediated by GATA-1- and NF-E2-binding proteins.  |  Boulanger, L., et al. 2002. J Biol Chem. 277: 41563-70. PMID: 12196550
4. Voltage-gated ion channels in the axon initial segment of human cortical pyramidal cells and their relationship with chandelier cells.  |  Inda, MC., et al. 2006. Proc Natl Acad Sci U S A. 103: 2920-5. PMID: 16473933
5. Axon initial segment cytoskeleton comprises a multiprotein submembranous coat containing sparse actin filaments.  |  Jones, SL., et al. 2014. J Cell Biol. 205: 67-81. PMID: 24711503
6. Functional diversity among spectrin isoforms.  |  Coleman, TR., et al. 1989. Cell Motil Cytoskeleton. 12: 225-47. PMID: 2655937
7. Two distinct variants of erythrocyte spectrin beta IV domain.  |  Pothier, B., et al. 1989. Hum Genet. 83: 373-6. PMID: 2807277
8. The spectrin network as a barrier to lateral diffusion in erythrocytes. A percolation analysis.  |  Saxton, MJ. 1989. Biophys J. 55: 21-8. PMID: 2930822
9. The present status of erythrocyte spectrin structure: the 106-residue repetitive structure is a basic feature of an entire class of proteins.  |  Speicher, DW. 1986. J Cell Biochem. 30: 245-58. PMID: 3517024
10. Modulation of spectrin-actin assembly by erythrocyte adducin.  |  Gardner, K. and Bennett, V. Nature. 328: 359-62. PMID: 3600811
11. A calmodulin and alpha-subunit binding domain in human erythrocyte spectrin.  |  Sears, DE., et al. 1986. Biochim Biophys Acta. 870: 432-42. PMID: 3697360
12. A partial structural repeat forms the heterodimer self-association site of all beta-spectrins.  |  Kennedy, SP., et al. 1994. J Biol Chem. 269: 11400-8. PMID: 8157672

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-spectrin β IV Antibody (F-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-spectrin β IV Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

spectrin β IV Antibody (F-5) Specifications

Alternate Product Name: spectrin β IV

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1661-1745

Epitope Target: spectrin β IV

Epitope Species: human

Product Reactivity/Detection: spectrin β IV isoforms 1, 3 and 4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for spectrin β IV Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin