spectrin α II Antibody (C-3): sc-48382

spectrin α II Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects spectrin α II protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). spectrin α II monoclonal antibody (C-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. spectrin α II, also known as fodrin α, plays a crucial role in maintaining the structural integrity of the cytoskeleton in non-erythroid cells, where spectrin α II is primarily located in the cytoplasm. spectrin α II is essential for the organization and stabilization of the cell membrane, as spectrin α II forms a mesh-like network that supports cellular shape and facilitates intracellular transport. The spectrin cytoskeletal network is vital for various cellular processes, including cell signaling, motility, and maintaining the mechanical properties of the cell. spectrin α II interacts with other cytoskeletal proteins, such as actin and various membrane proteins, to form a dynamic and adaptable structure that responds to cellular needs. The breakdown of spectrin α II by calpain activation can lead to significant alterations in cellular architecture, highlighting spectrin α II′s importance in maintaining cellular homeostasis and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

spectrin α II Antibody (C-3) References:

1. Selective release of calpain produced alphalI-spectrin (alpha-fodrin) breakdown products by acute neuronal cell death.  |  Dutta, S., et al. 2002. Biol Chem. 383: 785-91. PMID: 12108543
2. Spectrin alpha II and beta II isoforms interact with high affinity at the tetramerization site.  |  Bignone, PA. and Baines, AJ. 2003. Biochem J. 374: 613-24. PMID: 12820899
3. Characterization and expression of a heart-selective alternatively spliced variant of alpha II-spectrin, cardi+, during development in the rat.  |  Zhang, Y., et al. 2010. J Mol Cell Cardiol. 48: 1050-9. PMID: 20114050
4. Functional diversity among spectrin isoforms.  |  Coleman, TR., et al. 1989. Cell Motil Cytoskeleton. 12: 225-47. PMID: 2655937
5. The spectrin network as a barrier to lateral diffusion in erythrocytes. A percolation analysis.  |  Saxton, MJ. 1989. Biophys J. 55: 21-8. PMID: 2930822
6. Comparison of nonerythroid alpha-spectrin genes reveals strict homology among diverse species.  |  Leto, TL., et al. 1988. Mol Cell Biol. 8: 1-9. PMID: 3336352
7. The present status of erythrocyte spectrin structure: the 106-residue repetitive structure is a basic feature of an entire class of proteins.  |  Speicher, DW. 1986. J Cell Biochem. 30: 245-58. PMID: 3517024
8. Modulation of spectrin-actin assembly by erythrocyte adducin.  |  Gardner, K. and Bennett, V. Nature. 328: 359-62. PMID: 3600811
9. Progressive Ataxia, Memory Impairments, and Seizure Episodes in Spna2 R1098Q Mouse Variant Affecting Alpha II Spectrin's Scaffold Stability.  |  Zalas, M., et al. 2023. Brain Sci. 13: PMID: 36831804
10. Erk Inhibition as a Promising Therapeutic Strategy for High IL-8-Secreting and Low SPTAN1-Expressing Colorectal Cancer.  |  Meier, C., et al. 2024. Int J Mol Sci. 25: PMID: 38891846
11. Abnormal spectrin in hereditary elliptocytosis.  |  Marchesi, SL., et al. 1986. Blood. 67: 141-51. PMID: 3940543
12. A partial structural repeat forms the heterodimer self-association site of all beta-spectrins.  |  Kennedy, SP., et al. 1994. J Biol Chem. 269: 11400-8. PMID: 8157672

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-spectrin α II Antibody (C-3) has 42 citations in a variety of scientific publications.

Primary antibodies like Anti-spectrin α II Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

spectrin α II Antibody (C-3) Specifications

Alternate Product Name: spectrin α II

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2368-2472

Epitope Target: spectrin α II

Epitope Species: human

Product Reactivity/Detection: spectrin α II

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 42 citations in various scientific publications

Available Conjugates/Forms for spectrin α II Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin