Sp3 Antibody (G-7): sc-365220

Sp3 Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sp3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Sp3 (G-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Sp transcription factor family, which includes Sp1, Sp2, Sp3, and Sp4, is characterized by a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus, allowing these proteins to bind to specific DNA sequences known as GC boxes. Sp3 interaction with GC boxes is crucial for regulating gene expression, playing a significant role in transcriptional activation and repression of target genes. Sp3 stands unique among family members by inhibiting transcriptional activity of Sp1 and Sp4, thereby fine-tuning gene expression involved in various cellular processes. Multiple isoforms of Sp3 arise from alternative splicing, adding another layer of complexity to regulatory functions. The diverse expression patterns of Sp family members, particularly the ubiquitous presence of Sp1, Sp2, and Sp3 compared to the more restricted expression of Sp4, highlight Sp3′s importance in a wide range of biological contexts, making anti-Sp3 antibody (G-7) an essential tool for studying gene regulation and transcriptional dynamics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Sp3 Antibody (G-7) References:

1. Nuclear organization and chromatin dynamics--Sp1, Sp3 and histone deacetylases.  |  Davie, JR., et al. 2008. Adv Enzyme Regul. 48: 189-208. PMID: 18187045
2. The p65 subunit of NF-κB inhibits COL1A1 gene transcription in human dermal and scleroderma fibroblasts through its recruitment on promoter by protein interaction with transcriptional activators (c-Krox, Sp1, and Sp3).  |  Beauchef, G., et al. 2012. J Biol Chem. 287: 3462-78. PMID: 22139845
3. Sp1/Sp3 transcription factors regulate hallmarks of megakaryocyte maturation and platelet formation and function.  |  Meinders, M., et al. 2015. Blood. 125: 1957-67. PMID: 25538045
4. Automated sample preparation with SP3 for low-input clinical proteomics.  |  Müller, T., et al. 2020. Mol Syst Biol. 16: e9111. PMID: 32129943
5. ZEB1 Mediates Fibrosis in Corneal Endothelial Mesenchymal Transition Through SP1 and SP3.  |  Lee, J., et al. 2020. Invest Ophthalmol Vis Sci. 61: 41. PMID: 32721022
6. SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*.  |  Yan, T., et al. 2021. Chembiochem. 22: 1841-1851. PMID: 33442901
7. A novel Cbx1, PurB, and Sp3 complex mediates long-term silencing of tissue- and lineage-specific genes.  |  Baksh, SS., et al. 2022. J Biol Chem. 298: 102053. PMID: 35605661
8. Sp3 is essential for normal lung morphogenesis and cell cycle progression during mouse embryonic development.  |  McCoy, AM., et al. 2023. Development. 150: PMID: 36762637
9. LINC00115 regulates lung adenocarcinoma progression via sponging miR-154-3p to modulate Sp3 expression.  |  Sun, K., et al. 2023. Mol Cell Probes. 68: 101909. PMID: 36889558
10. Gel-Based Sample Fractionation with SP3-Purification for Top-Down Proteomics.  |  Takemori, A., et al. 2025. J Proteome Res.. PMID: 39841590

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sp3 Antibody (G-7) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Sp3 Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sp3 Antibody (G-7) Specifications

Alternate Product Name: Transcription Factor SP3

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 627-651

Epitope Target: Sp3

Epitope Species: human

Product Reactivity/Detection: Sp3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Sp3 Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Sp3 antibody is Sp3 Antibody (F-7): sc-28305.