Sp3 Antibody (D-6): sc-365038

Sp3 Antibody (D-6) is a mouse monoclonal IgG2a antibody that detects Sp3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Sp3 is a member of the Sp transcription factor family, which also includes Sp1, Sp2, and Sp4, and is characterized by a highly conserved DNA-binding domain featuring three zinc fingers at its C-terminus. This structural feature is crucial as Sp3 binds to specific DNA sequences known as GC boxes, which are essential for the regulation of gene expression. Notably, Sp3 plays a unique role in transcriptional regulation of various genes, including those involved in neuronal function, by inhibiting the activity of other Sp family members like Sp1 and Sp4. Sp3′s ability to modulate gene expression is particularly important in the context of neuronal nicotinic acetylcholine receptors, where Sp3 interacts with regulatory elements that influence receptor subunit expression. Additionally, multiple isoforms of Sp3, resulting from alternative splicing, add another layer of complexity to regulatory functions, making Sp3 a significant player in cellular processes and a valuable target for research in gene regulation and neurobiology.

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Sp3 Antibody (D-6) References:

1. Sp1 and Sp3 are oxidative stress-inducible, antideath transcription factors in cortical neurons.  |  Ryu, H., et al. 2003. J Neurosci. 23: 3597-606. PMID: 12736330
2. Transcription factor Sp3 knockout mice display serious cardiac malformations.  |  van Loo, PF., et al. 2007. Mol Cell Biol. 27: 8571-82. PMID: 17923686
3. Transcription factors Sp1 and Sp3 regulate expression of human ABCG2 gene and chemoresistance phenotype.  |  Yang, WJ., et al. 2013. Mol Cells. 36: 368-75. PMID: 23996530
4. Nickel or phenanthroline mediated intramolecular arylation of sp3 C-H bonds using aryl halides.  |  Wertjes, WC., et al. 2013. Org Lett. 15: 5986-9. PMID: 24256509
5. Extending the Compatibility of the SP3 Paramagnetic Bead Processing Approach for Proteomics.  |  Moggridge, S., et al. 2018. J Proteome Res. 17: 1730-1740. PMID: 29565595
6. Robust hematopoietic specification requires the ubiquitous Sp1 and Sp3 transcription factors.  |  Gilmour, J., et al. 2019. Epigenetics Chromatin. 12: 33. PMID: 31164147
7. Nrf2-regulated miR-380-3p Blocks the Translation of Sp3 Protein and Its Mediation of Paraquat-Induced Toxicity in Mouse Neuroblastoma N2a Cells.  |  Cai, Z., et al. 2019. Toxicol Sci. 171: 515-529. PMID: 31368498
8. Genomics and cellulolytic, hemicellulolytic, and amylolytic potential of Iocasia fonsfrigidae strain SP3-1 for polysaccharide degradation.  |  Heng, S., et al. 2022. PeerJ. 10: e14211. PMID: 36281362
9. Evaluation of SP3 for antibody-free quantification of tau in CSF mimic and brain by mass spectrometry.  |  Jacquemin, C., et al. 2024. Eur J Mass Spectrom (Chichester). 30: 65-75. PMID: 38258392
10. Transcription factor Sp3 antagonizes activation of the ornithine decarboxylase promoter by Sp1.  |  Kumar, AP. and Butler, AP. 1997. Nucleic Acids Res. 25: 2012-9. PMID: 9115370

The preferred Sp3 antibody is Sp3 Antibody (F-7): sc-28305.