Smt3 Antibody (D-8): sc-137177

Smt3 Antibody (D-8) is a mouse monoclonal IgG2b antibody that detects Smt3 in Saccharomyces cerevisiae by applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Smt3, a ubiquitin-like protein, plays a crucial role in cellular processes by modifying target proteins through a process known as SUMOylation, which is essential for regulating various cellular functions, including transcription, DNA repair, and cell cycle progression. The ability of Smt3 to conjugate to other proteins is vital for maintaining cellular homeostasis and responding to stress, as Smt3 helps modulate protein interactions and stability. Smt3 requires the E2 conjugating enzyme Ubc9 for attachment to substrates, and this modification can influence the localization and activity of target proteins, thereby impacting critical signaling pathways. Anti-Smt3 antibody (D-8) serves as an invaluable tool for researchers studying the dynamics of protein modification and the intricate networks of protein interactions in yeast, providing insights into the fundamental mechanisms of cellular regulation.

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Smt3 Antibody (D-8) References:

1. SUMO conjugation and deconjugation.  |  Schwienhorst, I., et al. 2000. Mol Gen Genet. 263: 771-86. PMID: 10905345
2. Posttranslational modification of TEL and TEL/AML1 by SUMO-1 and cell-cycle-dependent assembly into nuclear bodies.  |  Chakrabarti, SR., et al. 2000. Proc Natl Acad Sci U S A. 97: 13281-5. PMID: 11078523
3. SUMO keeps a check on recombination during DNA replication.  |  Ulrich, HD., et al. 2005. Cell Cycle. 4: 1699-702. PMID: 16294012
4. Noncovalent binding of small ubiquitin-related modifier (SUMO) protease to SUMO is necessary for enzymatic activities and cell growth.  |  Ihara, M., et al. 2007. J Biol Chem. 282: 16465-75. PMID: 17428805
5. Role of Cdc48/p97 as a SUMO-targeted segregase curbing Rad51-Rad52 interaction.  |  Bergink, S., et al. 2013. Nat Cell Biol. 15: 526-32. PMID: 23624404
6. NSMCE2 suppresses cancer and aging in mice independently of its SUMO ligase activity.  |  Jacome, A., et al. 2015. EMBO J. 34: 2604-19. PMID: 26443207
7. The Regulation of Chromatin by Dynamic SUMO Modifications.  |  Wilson, NR. and Hochstrasser, M. 2016. Methods Mol Biol. 1475: 23-38. PMID: 27631795
8. Engineered SUMO/protease system identifies Pdr6 as a bidirectional nuclear transport receptor.  |  Vera Rodriguez, A., et al. 2019. J Cell Biol. 218: 2006-2020. PMID: 31023724
9. SUMO and cellular adaptive mechanisms.  |  Ryu, HY., et al. 2020. Exp Mol Med. 52: 931-939. PMID: 32591648
10. Stability of Rad51 recombinase and persistence of Rad51 DNA repair foci depends on post-translational modifiers, ubiquitin and SUMO.  |  Antoniuk-Majchrzak, J., et al. 2023. Biochim Biophys Acta Mol Cell Res. 1870: 119526. PMID: 37364618

The preferred Smt3 antibody is Smt3 Antibody (E-1): sc-137158.