Smt3 Antibody (E-1): sc-137158

Smt3 Antibody (E-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Smt3 protein of Saccharomyces cerevisiae origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Smt3 antibody (E-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Smt3, a member of the ubiquitin-like protein family, plays a crucial role in cellular processes by regulating protein stability and localization through sumoylation, which maintains cellular homeostasis. This post-translational modification involves covalent attachment of Smt3 to target proteins, influencing function, interactions, and degradation pathways. Smt3 modifies proteins essential for DNA repair, cell cycle regulation, and stress responses, maintaining cellular integrity and function. Smt3 also interacts with several key proteins, including Ubc9, necessary for conjugation to substrates, and Skp1, which links cell cycle regulators to ubiquitin proteolysis machinery, highlighting Smt3′s integral role in cellular signaling and regulation.

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Smt3 Antibody (E-1) References:

1. Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway.  |  Gong, L. and Yeh, ET. 1999. J Biol Chem. 274: 12036-42. PMID: 10207026
2. Heat-induced cell cycle arrest of Saccharomyces cerevisiae: involvement of the RAD6/UBC2 and WSC2 genes in its reversal.  |  Raboy, B., et al. 1999. Mol Microbiol. 32: 729-39. PMID: 10361277
3. Characterization of a fission yeast SUMO-1 homologue, pmt3p, required for multiple nuclear events, including the control of telomere length and chromosome segregation.  |  Tanaka, K., et al. 1999. Mol Cell Biol. 19: 8660-72. PMID: 10567589
4. UBC13, a DNA-damage-inducible gene, is a member of the error-free postreplication repair pathway in Saccharomyces cerevisiae.  |  Brusky, J., et al. 2000. Curr Genet. 37: 168-74. PMID: 10794173
5. Isolation and characterization of HRT1 using a genetic screen for mutants unable to degrade Gic2p in saccharomyces cerevisiae.  |  Blondel, M., et al. 2000. Genetics. 155: 1033-44. PMID: 10880467
6. SUMO conjugation regulates immune signalling.  |  Hegde, S., et al. 2020. Fly (Austin). 14: 62-79. PMID: 32777975
7. Ubiquitin, proteasomes, and the regulation of intracellular protein degradation.  |  Hochstrasser, M. 1995. Curr Opin Cell Biol. 7: 215-23. PMID: 7612274
8. The ubiquitin-proteasome proteolytic pathway.  |  Ciechanover, A. 1994. Cell. 79: 13-21. PMID: 7923371
9. The ubiquitin-mediated proteolytic pathway: mechanisms of recognition of the proteolytic substrate and involvement in the degradation of native cellular proteins.  |  Ciechanover, A. and Schwartz, AL. 1994. FASEB J. 8: 182-91. PMID: 8119489
10. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box.  |  Bai, C., et al. 1996. Cell. 86: 263-74. PMID: 8706131
11. The ubiquitin-like proteins SMT3 and SUMO-1 are conjugated by the UBC9 E2 enzyme.  |  Schwarz, SE., et al. 1998. Proc Natl Acad Sci U S A. 95: 560-4. PMID: 9435231
12. A novel protein modification pathway related to the ubiquitin system.  |  Liakopoulos, D., et al. 1998. EMBO J. 17: 2208-14. PMID: 9545234

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Smt3 Antibody (E-1) has 1 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Smt3 Antibody (E-1) Specifications

Alternate Product Name: Smt3

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 15-98

Epitope Target: Smt3

Epitope Species: Saccharomyces cerevisiae

Product Reactivity/Detection: Smt3

Species Reactivity/Detection: y

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Smt3 Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin