Smad3 Antibody (4C9): sc-517574

Smad3 Antibody (4C9) is a mouse monoclonal IgG antibody meticulously developed to specifically target and detect Smad3 protein of human origin, exhibiting robust reactivity with Smad3 from mouse, rat, and human species in both immunofluorescence (IF) and immunohistochemistry with paraffin-embedded sections (IHCP) applications. Smad3, also referred to as LDS3, mad3, LDS1C, MADH3, JV15-2, hMAD-3, hSMAD3, HSPC193, and HsT17436, is a critical component of the Transforming Growth Factor-beta (TGFβ) signaling pathway, playing an essential role in regulating gene expression that governs cell proliferation, differentiation, apoptosis, and extracellular matrix production. Structurally, Smad3 is composed of distinct domains, including the MH1 (Mad Homology 1) domain responsible for binding to DNA, the MH2 domain that facilitates interactions with other proteins, and a linker region that modulates activity and interactions with transcriptional co-regulators. This unique structural configuration allows Smad3 to effectively transduce signals from the cell membrane to the nucleus, where Smad3 influences the transcription of target genes critical for maintaining cellular homeostasis and proper tissue development. The integrity of Smad3′s structure is paramount for function, as any alterations can disrupt the ability to regulate gene expression, leading to various pathological conditions such as fibrosis, cancer progression, and impaired immune responses. By providing reliable and specific detection of Smad3, anti-Smad3 antibody (4C9) enables researchers to explore the intricate mechanisms of TGF-β signaling and the structural-functional relationships of Smad3, thereby facilitating advancements in biomedical research and the development of targeted therapeutic strategies.

The preferred Smad3 antibody is Smad3 Antibody (38-Q): sc-101154.