Six1 Antibody (B-8): sc-514441
- Six1 Antibody (B-8) is a mouse monoclonal IgG2a κ Six1 antibody, cited in 7 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 251-274 near the C-terminus of Six1 of human origin
- Six1 Antibody (B-8) is recommended for detection of Six1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Six1 Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-514441 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Six1 Antibody (B-8) for WB applications. These reagents are now offered in bundles with Six1 Antibody (B-8) (see ordering information below).
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Six1 Antibody (B-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects Six1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Six1 (B-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Six1, a member of the sine oculis homeobox family of transcription factors, plays a crucial role in developmental processes and cellular differentiation. Six1 protein′s ability to regulate gene expression is vital for proper embryonic development, particularly in muscle and kidney formation. Notably, Six1 expression is typically low or absent in normal adult tissues but is found to be overexpressed in several tumor types, including breast carcinoma. This aberrant expression can disrupt normal cell cycle regulation, specifically by abrogating the G2 cell cycle checkpoint, which may contribute to tumor progression. Six1 interaction with other transcription factors, such as SIX4, further underscores its importance in gene regulation and highlights the potential for targeting this pathway in cancer therapies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Six1 Antibody (B-8) References:
- HOX genes in human cancers. | Cillo, C. Invasion Metastasis. 14: 38-49. PMID: 7657531
- Defective G2 checkpoint function in cells from individuals with familial cancer syndromes. | Paules, RS., et al. 1995. Cancer Res. 55: 1763-73. PMID: 7712486
- Identification and expression of six family genes in mouse retina. | Kawakami, K., et al. 1996. FEBS Lett. 393: 259-63. PMID: 8814301
- Fission yeast rad12+ regulates cell cycle checkpoint control and is homologous to the Bloom's syndrome disease gene. | Davey, S., et al. 1998. Mol Cell Biol. 18: 2721-8. PMID: 9566891
- Abrogation of the G2 cell cycle checkpoint associated with overexpression of HSIX1: a possible mechanism of breast carcinogenesis. | Ford, HL., et al. 1998. Proc Natl Acad Sci U S A. 95: 12608-13. PMID: 9770533
- Expression of myogenin during embryogenesis is controlled by Six/sine oculis homeoproteins through a conserved MEF3 binding site. | Spitz, F., et al. 1998. Proc Natl Acad Sci U S A. 95: 14220-5. PMID: 9826681
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Six1 Antibody (B-8) | sc-514441 | 200 µg/ml | $322.00 | |||
Six1 Antibody (B-8): m-IgG Fc BP-HRP Bundle | sc-531128 | 200 µg Ab; 10 µg BP | $361.00 | |||
Six1 Antibody (B-8): m-IgGκ BP-HRP Bundle | sc-524727 | 200 µg Ab, 40 µg BP | $361.00 | |||
Six1 Antibody (B-8): m-IgG2a BP-HRP Bundle | sc-547695 | 200 µg Ab; 10 µg BP | $361.00 | |||
Six1 Antibody (B-8) X | sc-514441 X | 200 µg/0.1 ml | $322.00 | |||
Six1 Antibody (B-8) AC | sc-514441 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Six1 Antibody (B-8) HRP | sc-514441 HRP | 200 µg/ml | $322.00 | |||
Six1 Antibody (B-8) FITC | sc-514441 FITC | 200 µg/ml | $336.00 | |||
Six1 Antibody (B-8) PE | sc-514441 PE | 200 µg/ml | $349.00 | |||
Six1 Antibody (B-8) Alexa Fluor® 488 | sc-514441 AF488 | 200 µg/ml | $364.00 | |||
Six1 Antibody (B-8) Alexa Fluor® 546 | sc-514441 AF546 | 200 µg/ml | $364.00 | |||
Six1 Antibody (B-8) Alexa Fluor® 594 | sc-514441 AF594 | 200 µg/ml | $364.00 | |||
Six1 Antibody (B-8) Alexa Fluor® 647 | sc-514441 AF647 | 200 µg/ml | $364.00 | |||
Six1 Antibody (B-8) Alexa Fluor® 680 | sc-514441 AF680 | 200 µg/ml | $364.00 | |||
Six1 Antibody (B-8) Alexa Fluor® 790 | sc-514441 AF790 | 200 µg/ml | $364.00 | |||
Six1 (B-8) Neutralizing Peptide | sc-514441 P | 100 µg/0.5 ml | $69.00 |