SIRT3 Antibody (F-10) recommended for detection of SIRT3 of mouse, rat and human origin by WB, IP, IF and ELISA

SIRT3 Antibody (F-10): sc-365175

SIRT3 Antibody (F-10) is rated 4.4 out of 5 by 21.
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Datasheets

    • Anti-SIRT3 Antibody (F-10) is a mouse monoclonal IgG2b (kappa light chain) SIRT3 antibody provided at 200 µg/ml
    • raised against amino acids 251-290 mapping within an internal region of SIRT3 of human origin
    • Anti-SIRT3 Antibody (F-10) is recommended for detection of SIRT3 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-SIRT3 Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SIRT3 (F-10): sc-365175.

Does this works for immunoprecipitation? How much Antibody would I need for IP? Is there specific protocol for IP using agarose conjugated antibody?

Asked by: Needs
Thank you for your question. Here is a link to our recommended IP protocol: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots The recommended starting conditions are in the protocol, which covers both conjugated and non-conjugated antibody conditions. Please contact technical service if you need further assistance.
Answered by: Tech Service
Date published: 2019-01-28

Does this antibody cross react with murine SIRT3?

Asked by: JoshP
Thank you for your question. SIRT3 Antibody (F-10): sc-365175 recommended for detection of SIRT3 of mouse, rat and human origin by WB, IP, IF and ELISA, so it will detect SIRT3 of murine origin. For further information please contact our technical service directly.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What is the recommended secondary for this mouse monoclonal antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-01-30

I saw two bands in between 25 and 37. Are they both Sirt3?

Asked by: SuperChang
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Rated 2 out of 5 by from Señal muy bandeada. Aparecen varias bandas. Una de ellas en peso molecular. Difícil cuantificación de la señal.
Date published: 2020-05-09
Rated 5 out of 5 by from Great for ICC Used in human primary fibroblasts. Diluted 1:300 in PBST (0.1% triton) + 10% Serum. O/N 4ºC incubation.
Date published: 2020-02-19
Rated 3 out of 5 by from Good antibody We used this antibody on human monocytoid cells THP-1 activated with 100 nM PMA for 72 hours.
Date published: 2019-01-31
Rated 5 out of 5 by from Good Antibody Gave very strong bands for WB when used at 1.500 for various mouse tissue samples (60ug of protein loaded per well). Also worked for WB when staining for sirt3 in human dermal fibroblast samples at 1.500 (20ug protein loaded per well), although staining was usually much fainter when diluted antibody was used for second and third time. Will buy again.
Date published: 2018-11-24
Rated 1 out of 5 by from It did not work for us. We acquired this antibody about 7 months ago and performed whole cell lysates on HeLa cells. We used this antibody at a 1 to 1000 dilution and could not detect a band at all. We attempted this twice at the same conditions with no success. It should be noted that the condition mentioned was successful with several other SCBT antibodies.
Date published: 2018-06-14
Rated 5 out of 5 by from Great results for mytochondrial protein This antibody works well in rat liver tissue. I used total protein extraction and I'm very satisfied with that results. I didn`t need work with the subcellular fraction. Dilution 1:2000
Date published: 2018-03-13
Rated 5 out of 5 by from excellent for WB The antibody worked well for WB mouse endogenous protein at 1:1000 dilution in 3% BSA, PBST.
Date published: 2018-02-15
Rated 4 out of 5 by from Good for IHC I have tried this SIRT3 antibody for IHC(1:100 dilution). It works well.
Date published: 2017-12-11
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