SIRT3 Antibody (F-10): sc-365175

SIRT3 Antibody (F-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects SIRT3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-SIRT3 antibody (F-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SIRT3 plays a crucial role in mitochondrial function as a NAD-dependent deacetylase, which is essential for regulating metabolic processes and maintaining cellular homeostasis. By deacetylating specific lysine residues on target proteins within the mitochondria, SIRT3 modulates various pathways, including those involved in energy metabolism and oxidative stress response. This regulation is vital for cellular adaptation to stress and has implications in aging and age-related diseases, making SIRT3 a significant focus of research in the context of metabolic disorders and mitochondrial dysfunction. SIRT3 is processed by mitochondrial processing peptidase to yield its active form, highlighting the importance of post-translational modifications in functional activity.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SIRT3 Antibody (F-10) References:

1. Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity.  |  Frye, RA. 1999. Biochem Biophys Res Commun. 260: 273-9. PMID: 10381378
2. Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins.  |  Frye, RA. 2000. Biochem Biophys Res Commun. 273: 793-8. PMID: 10873683
3. Identification of a class of small molecule inhibitors of the sirtuin family of NAD-dependent deacetylases by phenotypic screening.  |  Grozinger, CM., et al. 2001. J Biol Chem. 276: 38837-43. PMID: 11483616
4. SIRT3, a mitochondrial sirtuin deacetylase, regulates mitochondrial function and thermogenesis in brown adipocytes.  |  Shi, T., et al. 2005. J Biol Chem. 280: 13560-7. PMID: 15653680
5. A novel VNTR enhancer within the SIRT3 gene, a human homologue of SIR2, is associated with survival at oldest ages.  |  Bellizzi, D., et al. 2005. Genomics. 85: 258-63. PMID: 15676284
6. Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins.  |  Michishita, E., et al. 2005. Mol Biol Cell. 16: 4623-35. PMID: 16079181
7. SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism.  |  Wang, T., et al. 2019. Mol Cell. 75: 823-834.e5. PMID: 31302001
8. Sirt3-mediated mitophagy regulates AGEs-induced BMSCs senescence and senile osteoporosis.  |  Guo, Y., et al. 2021. Redox Biol. 41: 101915. PMID: 33662874
9. SIRT3 consolidates heterochromatin and counteracts senescence.  |  Diao, Z., et al. 2021. Nucleic Acids Res. 49: 4203-4219. PMID: 33706382
10. The Role of SIRT3 in the Osteoporosis.  |  Hu, S. and Wang, S. 2022. Front Endocrinol (Lausanne). 13: 893678. PMID: 35692409
11. The Role of SIRT3 in Exercise and Aging.  |  Zhou, L., et al. 2022. Cells. 11: PMID: 36010672
12. SIRT3-dependent delactylation of cyclin E2 prevents hepatocellular carcinoma growth.  |  Jin, J., et al. 2023. EMBO Rep. 24: e56052. PMID: 36896611

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SIRT3 Antibody (F-10) has 70 citations in a variety of scientific publications.

Primary antibodies like Anti-SIRT3 Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SIRT3 Antibody (F-10) Specifications

Alternate Product Name: SIRT3

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 251-290

Epitope Target: SIRT3

Epitope Species: human

Product Reactivity/Detection: SIRT3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 70 citations in various scientific publications

Available Conjugates/Forms for SIRT3 Antibody (F-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin