SIRT2 Antibody (A-5): sc-28298

SIRT2 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody specifically targeting SIRT2, also known as Sirtuin 2, a member of the silent information regulator (SIR2) family, which is conserved across species and plays critical roles in cellular processes such as transcriptional regulation, DNA repair, and aging. SIRT2, characterized by its NAD-dependent deacetylase activity, modulates various cellular functions, including cell cycle regulation and response to DNA damage, making it crucial for maintaining cellular health and genomic stability. anti-SIRT2 antibody (A-5) is raised against amino acids 1-95 of the human SIRT2 protein and demonstrates reactivity in mouse, rat, and human samples, making it versatile for use in applications like western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). SIRT2′s catalytic core includes a 323 amino acid NAD-binding domain and a large groove essential for its enzymatic function, facilitating mono-ADP-ribosylation and influencing protein interactions within the cell. Alongside its central role in deacetylating histones and regulating transcription at specific genetic loci, SIRT2 is part of a broader network of seven sirtuins (SIRT1-7) divided into four classes based on structure and function. SIRT2 (A-5) antibody is available in multiple conjugated forms for enhanced experimental flexibility, including options like agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® conjugates.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SIRT2 Antibody (A-5) References:

1. Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity.  |  Frye, RA. 1999. Biochem Biophys Res Commun. 260: 273-9. PMID: 10381378
2. The conserved core of a human SIR2 homologue functions in yeast silencing.  |  Sherman, JM., et al. 1999. Mol Biol Cell. 10: 3045-59. PMID: 10473645
3. Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins.  |  Frye, RA. 2000. Biochem Biophys Res Commun. 273: 793-8. PMID: 10873683
4. Sound silencing: the Sir2 protein and cellular senescence.  |  Defossez, PA., et al. 2001. Bioessays. 23: 327-32. PMID: 11268038
5. Structure of the histone deacetylase SIRT2.  |  Finnin, MS., et al. 2001. Nat Struct Biol. 8: 621-5. PMID: 11427894
6. Identification of a class of small molecule inhibitors of the sirtuin family of NAD-dependent deacetylases by phenotypic screening.  |  Grozinger, CM., et al. 2001. J Biol Chem. 276: 38837-43. PMID: 11483616
7. SIRT2: Controversy and multiple roles in disease and physiology.  |  Wang, Y., et al. 2019. Ageing Res Rev. 55: 100961. PMID: 31505260
8. Sirtuin 2 (SIRT2): Confusing Roles in the Pathophysiology of Neurological Disorders.  |  Chen, X., et al. 2021. Front Neurosci. 15: 614107. PMID: 34108853
9. Restoring nuclear entry of Sirtuin 2 in oligodendrocyte progenitor cells promotes remyelination during ageing.  |  Ma, XR., et al. 2022. Nat Commun. 13: 1225. PMID: 35264567
10. SIRT2 regulates extracellular vesicle-mediated liver-bone communication.  |  Lin, L., et al. 2023. Nat Metab. 5: 821-841. PMID: 37188819
11. Sirtuin 2 deficiency aggravates ageing-induced vascular remodelling in humans and mice.  |  Zhang, Y., et al. 2023. Eur Heart J. 44: 2746-2759. PMID: 37377116
12. SIRT2 negatively regulates the cGAS-STING pathway by deacetylating G3BP1.  |  Li, Y., et al. 2023. EMBO Rep. 24: e57500. PMID: 37870259

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SIRT2 Antibody (A-5) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-SIRT2 Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SIRT2 Antibody (A-5) Specifications

Alternate Product Name: SIRT2

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-95

Epitope Target: SIRT2

Epitope Species: human

Product Reactivity/Detection: SIRT2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for SIRT2 Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin