SIRT2 Antibody (A-5) recommended for detection of SIRT2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

SIRT2 Antibody (A-5): sc-28298

SIRT2 Antibody (A-5) is rated 4.1 out of 5 by 18.
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Datasheets

    • Anti-SIRT2 Antibody (A-5) is a mouse monoclonal IgG1 (kappa light chain) SIRT2 antibody provided at 200 µg/ml
    • raised against amino acids 1-95 of SIRT2 of human origin
    • Anti-SIRT2 Antibody (A-5) is recommended for detection of SIRT2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-SIRT2 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SIRT2 (A-5): sc-28298.

Is this antibody able to stain immature oligodendrocytes in brain tissue (paraffin embedded)?

Asked by: Alexandra333
Thank you for your question. Yes, this antibody is recommended and warrantied for IHC on paraffin sections. SIRT2 does appear to be expressed in brain, so it should be fine for brain tissue use.
Answered by: Tech Service
Date published: 2020-01-10

What is the recommended secondary for this antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2017-01-11

Can this antibody be used to stain mouse tissue in IF or IHC?

Asked by: AbPolly
Thank you for your question. Yes, it is appropriate for staining with mouse tissues. In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody SIRT2 Antibody (A-5): sc-28298 is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 6
Date published: 2017-01-11
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Rated 1 out of 5 by from No detecta la proteina. Aparece una banda que no corresponde al peso molecular de la proteina.
Date published: 2020-05-09
Rated 5 out of 5 by from SIRT2抗体很好用 SIRT2抗体非常好用,买过来做IP的,效果不错。另外,做WB效果也挺好。符合期刊审稿标准,已用该抗体发表多篇论文
Date published: 2020-03-22
Rated 2 out of 5 by from いまいち? WBで内在性のものを検出しようと試みましたが、ノンスペシフィックバンドらしきものしか検出されませんでした(発現抑制したレーンでバンドが消失していなかった)。publishされている論文でも使われていただけに少し残念です。過剰発現したものに当たるかはわかりません。
Date published: 2018-07-18
Rated 5 out of 5 by from Good Antibody SIRT2 Antibody is our ideal choice to do drugtest. Because the antibody with a precise position effect and very powerful signal.
Date published: 2017-10-21
Rated 5 out of 5 by from 功能很出色,效果非常明显 之前申请试用了santa的SIRT2抗体,感觉效果特别的好,实验结果比以前使用的抗体好很多,跑出来的条带很清晰很好看,打算继续购再购买一支。一直以来都很信任santa的产品,点赞!
Date published: 2017-10-08
Rated 5 out of 5 by from highly recommend The quality is very reliable detect SIRT2 by WB,1:800 diluted,clear band wasseen, highly recommend.
Date published: 2017-08-26
Rated 1 out of 5 by from 아예 밴드가 안떠요 Yeast로 Western blot을 하고있는데 베타액틴과 같은 위치에서 밴드가 잡혀야하는 데 잡히질 않네요 사진에서 위에 멤브레인에 sir2를 붙였구요 아래부분에는 베타액틴을 붙였어요 Stripping해서 반대로도 해보구요 베타액틴을 잘 붙는데 sir2는 22시간동안 4도에 두고 3시간 shaking incubate 했는 데도 밴드가 안잡혔어요 이전에도 몇번을 해봤는데도 안뜨더라구요
Date published: 2017-07-29
Rated 5 out of 5 by from good result on WB The SIRT2 antibody works well in Werstern Blot detection using Rat articular chondrocytes cell at 1:300. The protein size locates at 44kD . we got a clear protein band.
Date published: 2017-07-14
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