SHP Antibody (F-12): sc-271469

SHP Antibody (F-12) is a mouse monoclonal IgG2b antibody that detects SHP in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SHP, or short heterodimer partner, is an orphan nuclear receptor that plays a crucial role in regulating various metabolic processes, particularly in the liver, where SHP is predominantly expressed. SHP is unique among nuclear receptors as SHP contains dimerization and ligand-binding domains but lacks the conserved DNA-binding domain, which is essential for traditional receptor function. The absence of this domain allows SHP to act as a negative regulator of other nuclear receptors, including thyroid receptors and estrogen receptors, by inhibiting their DNA binding and competing for coactivator interactions. Additionally, SHP is involved in the modulation of NF-κB transcriptional activity, particularly in macrophages treated with oxidized low-density lipoprotein, highlighting SHP′s importance in inflammatory responses. The regulation of SHP is critical for maintaining cellular homeostasis, especially in the context of bile acid metabolism, where SHP serves as a protective factor against bile acid-induced damage in hepatocytes. By utilizing anti-SHP antibody (F-12), researchers can gain valuable insights into the intricate roles of SHP in metabolic regulation and potential implications in various diseases.

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SHP Antibody (F-12) References:

1. Short heterodimer partner (SHP) orphan nuclear receptor inhibits the transcriptional activity of aryl hydrocarbon receptor (AHR)/AHR nuclear translocator (ARNT).  |  Klinge, CM., et al. 2001. Arch Biochem Biophys. 390: 64-70. PMID: 11368516
2. Repression of CAR-mediated transactivation of CYP2B genes by the orphan nuclear receptor, short heterodimer partner (SHP).  |  Bae, Y., et al. 2004. DNA Cell Biol. 23: 81-91. PMID: 15000748
3. Small heterodimer partner (SHP) links hepatitis C and liver fibrosis: a small protein on the big stage.  |  Weiskirchen, R. and Tacke, F. 2015. Liver Int. 35: 2218-21. PMID: 26037155
4. Small heterodimer partner (SHP) contributes to insulin resistance in cardiomyocytes.  |  Rodríguez-Calvo, R., et al. 2017. Biochim Biophys Acta Mol Cell Biol Lipids. 1862: 541-551. PMID: 28214558
5. Small heterodimer partner (SHP) deficiency protects myocardia from lipid accumulation in high fat diet-fed mice.  |  Ohn, JH., et al. 2017. PLoS One. 12: e0186021. PMID: 29016649
6. Polygala saponins inhibit NLRP3 inflammasome-mediated neuroinflammation via SHP-2-Mediated mitophagy.  |  Qiu, WQ., et al. 2022. Free Radic Biol Med. 179: 76-94. PMID: 34933095
7. SHP-2 and PD-1-SHP-2 signaling regulate myeloid cell differentiation and antitumor responses.  |  Christofides, A., et al. 2023. Nat Immunol. 24: 55-68. PMID: 36581713
8. Consideration of SHP-1 as a Molecular Target for Tumor Therapy.  |  Lim, S., et al. 2023. Int J Mol Sci. 25: PMID: 38203502
9. SHP-1 inhibition targets leukaemia stem cells to restore immunosurveillance and enhance chemosensitivity by metabolic reprogramming.  |  Xu, X., et al. 2024. Nat Cell Biol. 26: 464-477. PMID: 38321204
10. Ablation of IFNγ in myeloid cells suppresses liver inflammation and fibrogenesis in mice with hepatic small heterodimer partner (SHP) deletion.  |  Zhu, L., et al. 2024. Mol Metab. 83: 101932. PMID: 38589002

The preferred SHP antibody is SHP Antibody (H-5): sc-271511.