Shc Antibody (F-7): sc-398163
- Shc Antibody (F-7) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 366-473 of Shc of human origin
- recommended for detection of Shc p66, p52 and p46 of mouse, rat and human origin by WB, IP, IF and ELISA
- See Shc (PG-797): sc-967 for Shc antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Shc Antibody (F-7) for WB applications. These reagents are now offered in bundles with Shc Antibody (F-7) (see ordering information below).
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Shc Antibody (F-7) is a mouse monoclonal IgG1 antibody that detects Shc in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Shc is a critical adapter protein that plays a pivotal role in signaling pathways activated by receptor tyrosine kinases, which are essential for various cellular responses, including growth and differentiation. Located primarily in the cytoplasm, Shc acts as a molecular bridge, linking activated receptors to downstream signaling molecules, thereby facilitating signal transmission to the nucleus. This function allows cells to respond appropriately to external growth signals, ensuring proper cellular function and homeostasis. Notably, Shc interacts with other key proteins, such as GRB2 and Sos, to activate the Ras signaling pathway, which is vital for cell proliferation and survival. Shc′s ability to form complexes with these proteins underscores its importance in insulin signaling and other growth factor-mediated pathways, making anti-Shc antibody (F-7) an invaluable tool for researchers studying these critical cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Shc Antibody (F-7) References:
- Hypoxia and HIF-1alpha stability: another stress-sensing mechanism for Shc. | Abe, J. and Berk, BC. 2002. Circ Res. 91: 4-6. PMID: 12114314
- Role of Shc in T-cell development and function. | Zhang, L., et al. 2003. Immunol Rev. 191: 183-95. PMID: 12614360
- p66(Shc) protein, oxidative stress, and cardiovascular complications of diabetes: the missing link. | Francia, P., et al. 2009. J Mol Med (Berl). 87: 885-91. PMID: 19590843
- Adapter protein Shc regulates Janus kinase 3 phosphorylation. | Mishra, J. and Kumar, N. 2014. J Biol Chem. 289: 15951-6. PMID: 24795043
- Modulation of Obesity and Insulin Resistance by the Redox Enzyme and Adaptor Protein p66Shc. | Ciciliot, S. and Fadini, GP. 2019. Int J Mol Sci. 20: PMID: 30813483
- TrkB-Shc Signaling Protects against Hippocampal Injury Following Status Epilepticus. | Huang, YZ., et al. 2019. J Neurosci. 39: 4624-4630. PMID: 30926745
- Targeting the Shc-EGFR interaction with indomethacin inhibits MAP kinase pathway signalling. | Lin, CC., et al. 2019. Cancer Lett. 457: 86-97. PMID: 31100409
- Diurnal oscillations of endogenous H2O2 sustained by p66Shc regulate circadian clocks. | Pei, JF., et al. 2019. Nat Cell Biol. 21: 1553-1564. PMID: 31768048
- The Shc protein Rai enhances T-cell survival under hypoxia. | Criscuoli, M., et al. 2020. J Cell Physiol. 235: 8058-8070. PMID: 31944299
- Shc inhibitor idebenone ameliorates liver injury and fibrosis in dietary NASH in mice. | Jiang, JX., et al. 2021. J Biochem Mol Toxicol. 35: e22876. PMID: 34369032
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Shc Antibody (F-7) | sc-398163 | 200 µg/ml | $322.00 | |||
Shc Antibody (F-7): m-IgG Fc BP-HRP Bundle | sc-538381 | 200 µg Ab; 10 µg BP | $361.00 | |||
Shc Antibody (F-7): m-IgGκ BP-HRP Bundle | sc-535986 | 200 µg Ab; 40 µg BP | $361.00 | |||
Shc Antibody (F-7): m-IgG1 BP-HRP Bundle | sc-545673 | 200 µg Ab; 20 µg BP | $361.00 |