SF2/ASF Antibody (96): sc-33652
- SF2/ASF Antibody (96) is a mouse monoclonal IgG2b κ SF2/ASF antibody, cited in 67 publications, provided at 200 µg/ml
- epitope mapping near the N-terminus of the SF2/ASF protein consisting of the RRM1 region
- SF2/ASF Antibody (96) is recommended for detection of SF2/ASF of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-SF2/ASF Antibody (96) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SF2/ASF Antibody (96) for WB and IHC(P) applications. These reagents are now offered in bundles with SF2/ASF Antibody (96) (see ordering information below).
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SF2/ASF Antibody (96) is a mouse monoclonal IgG2b kappa light chain antibody that detects SF2/ASF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-SF2/ASF antibody (96) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SF2/ASF protein, also known as Alternative Splicing Factor 1, plays a crucial role in regulating pre-mRNA splicing, which is essential for generating diverse protein isoforms from a single gene. SF2/ASF protein is primarily located in the nucleus, where binding to pre-mRNA facilitates splice site recognition, thereby influencing the splicing process. Proper localization of SF2/ASF protein ensures efficient splicing and subsequent mRNA export to the cytoplasm, where translation occurs. SF2/ASF protein interacts with other splicing factors, such as SC35 and SRp20, forming a complex network that fine-tunes the splicing machinery. Dysregulation of SF2/ASF protein and associated pathways can lead to various diseases, including cancer, highlighting SF2/ASF′s importance in maintaining cellular homeostasis and proper gene expression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SF2/ASF Antibody (96) References:
- The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and phosphorylation. | Petersen-Mahrt, SK., et al. 1999. EMBO J. 18: 1014-24. PMID: 10022843
- Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences. | Schaal, TD. and Maniatis, T. 1999. Mol Cell Biol. 19: 1705-19. PMID: 10022858
- The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. | Cavaloc, Y., et al. 1999. RNA. 5: 468-83. PMID: 10094314
- The splicing factor SF2/ASF regulates translation initiation by enhancing phosphorylation of 4E-BP1. | Michlewski, G., et al. 2008. Mol Cell. 30: 179-89. PMID: 18439897
- SF2/ASF autoregulation involves multiple layers of post-transcriptional and translational control. | Sun, S., et al. 2010. Nat Struct Mol Biol. 17: 306-12. PMID: 20139984
- The oncoprotein SF2/ASF promotes non-small cell lung cancer survival by enhancing survivin expression. | Ezponda, T., et al. 2010. Clin Cancer Res. 16: 4113-25. PMID: 20682707
- Enlargement of speckles of SF2/ASF due to loss of function of Smu1 is characterized in the mammalian temperature-sensitive mutant. | Sugaya, K., et al. 2011. RNA Biol. 8: 488-95. PMID: 21519198
- SF2/ASF binding region within JC virus NCCR limits early gene transcription in glial cells. | Uleri, E., et al. 2013. Virol J. 10: 147. PMID: 23672192
- Function of conserved domains of hnRNP A1 and other hnRNP A/B proteins. | Mayeda, A., et al. 1994. EMBO J. 13: 5483-95. PMID: 7957114
- Specific commitment of different pre-mRNAs to splicing by single SR proteins. | Fu, XD. 1993. Nature. 365: 82-5. PMID: 8361546
- The splicing factor SRp20 modifies splicing of its own mRNA and ASF/SF2 antagonizes this regulation. | Jumaa, H. and Nielsen, PJ. 1997. EMBO J. 16: 5077-85. PMID: 9305649
- A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm. | Cáceres, JF., et al. 1998. Genes Dev. 12: 55-66. PMID: 9420331
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SF2/ASF Antibody (96) | sc-33652 | 200 µg/ml | $316.00 | |||
SF2/ASF Antibody (96): m-IgG Fc BP-HRP Bundle | sc-528481 | 200 µg Ab; 10 µg BP | $354.00 | |||
SF2/ASF Antibody (96): m-IgGκ BP-HRP Bundle | sc-520871 | 200 µg Ab, 40 µg BP | $354.00 | |||
SF2/ASF Antibody (96): m-IgG2b BP-HRP Bundle | sc-550041 | 200 µg Ab; 10 µg BP | $354.00 | |||
SF2/ASF Antibody (96) AC | sc-33652 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SF2/ASF Antibody (96) HRP | sc-33652 HRP | 200 µg/ml | $316.00 | |||
SF2/ASF Antibody (96) FITC | sc-33652 FITC | 200 µg/ml | $330.00 | |||
SF2/ASF Antibody (96) PE | sc-33652 PE | 200 µg/ml | $343.00 | |||
SF2/ASF Antibody (96) Alexa Fluor® 488 | sc-33652 AF488 | 200 µg/ml | $357.00 | |||
SF2/ASF Antibody (96) Alexa Fluor® 546 | sc-33652 AF546 | 200 µg/ml | $357.00 | |||
SF2/ASF Antibody (96) Alexa Fluor® 594 | sc-33652 AF594 | 200 µg/ml | $357.00 | |||
SF2/ASF Antibody (96) Alexa Fluor® 647 | sc-33652 AF647 | 200 µg/ml | $357.00 | |||
SF2/ASF Antibody (96) Alexa Fluor® 680 | sc-33652 AF680 | 200 µg/ml | $357.00 | |||
SF2/ASF Antibody (96) Alexa Fluor® 790 | sc-33652 AF790 | 200 µg/ml | $357.00 |