SF2/ASF Antibody (3G268): sc-73026

SF2/ASF Antibody (3G268) is a mouse monoclonal IgG2b kappa light chain antibody that detects SF2/ASF in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-SF2/ASF antibody (3G268) is available in a non-conjugated form, allowing for versatile experimental use. SF2/ASF, also known as SRSF1, plays a critical role in pre-mRNA splicing by enhancing the recognition of weak splice sites, which is essential for accurate mRNA processing. SF2/ASF (3G268) antibody targets a protein primarily located in the nucleus, where SF2/ASF interacts with splicing enhancer elements—short RNA sequences that activate nearby weak splice sites. SF2/ASF presence in the nucleus is vital because SF2/ASF not only facilitates constitutive splicing of pre-mRNA but also regulates alternative splicing, which is crucial for generating diverse protein isoforms from a single gene. SF2/ASF shuttles between the nucleus and cytoplasm, contributing to mRNA transport, thereby influencing gene expression at multiple levels. SF2/ASF activity is finely tuned by interactions with other splicing factors, including members of the serine/arginine-rich (SR) protein family, which contain RNA recognition motifs and an RS-rich domain. Understanding SF2/ASF function and dynamics is essential for elucidating gene regulation complexities and potential implications in various diseases, including cancer, where splicing mechanisms may be disrupted.

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SF2/ASF Antibody (3G268) References:

1. Mapping the SF2/ASF binding sites in the bovine growth hormone exonic splicing enhancer.  |  Dirksen, WP., et al. 2000. J Biol Chem. 275: 29170-7. PMID: 10880506
2. Hypophosphorylated ASF/SF2 binds TAP and is present in messenger ribonucleoproteins.  |  Lai, MC. and Tarn, WY. 2004. J Biol Chem. 279: 31745-9. PMID: 15184380
3. SF2/ASF protein binds to the cap region of human topoisomerase I through two RRM domains.  |  Kowalska-Loth, B., et al. 2005. Biochem Biophys Res Commun. 331: 398-403. PMID: 15850773
4. Alternative splicing factor ASF/SF2 is down regulated in inflamed muscle.  |  Xiong, Z., et al. 2006. J Clin Pathol. 59: 855-61. PMID: 16574722
5. The serine/arginine-rich protein SF2/ASF regulates protein sumoylation.  |  Pelisch, F., et al. 2010. Proc Natl Acad Sci U S A. 107: 16119-24. PMID: 20805487
6. Treatment with IL-17 prolongs the half-life of chemokine CXCL1 mRNA via the adaptor TRAF5 and the splicing-regulatory factor SF2 (ASF).  |  Sun, D., et al. 2011. Nat Immunol. 12: 853-60. PMID: 21822258
7. JC polyomavirus expression and bell-shaped regulation of its SF2/ASF suppressor during the follow-up of multiple sclerosis patients treated with natalizumab.  |  Uleri, E., et al. 2017. J Neurovirol. 23: 226-238. PMID: 27812788
8. Regulation of the Alternative Splicing and Function of Cyclin T1 by the Serine-Arginine-Rich Protein ASF/SF2.  |  Zhou, J., et al. 2017. J Cell Biochem. 118: 4020-4032. PMID: 28422315
9. miR‑30c suppresses prostate cancer survival by targeting the ASF/SF2 splicing factor oncoprotein.  |  Huang, YQ., et al. 2017. Mol Med Rep. 16: 2431-2438. PMID: 28677791
10. General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer.  |  Sun, Q., et al. 1993. Genes Dev. 7: 2598-608. PMID: 8276242

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SF2/ASF Antibody (3G268) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-SF2/ASF Antibody (3G268) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SF2/ASF Antibody (3G268) Specifications

Alternate Product Name: SRSF1

Product Clone Name: 3G268

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: SF2/ASF

Epitope Species:

Product Reactivity/Detection: SF2/ASF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for SF2/ASF Antibody (3G268): N/A

The preferred SF2/ASF antibody is SF2/ASF Antibody (96): sc-33652.