RYBP Antibody (A-1): sc-374235
- RYBP Antibody (A-1) is a mouse monoclonal IgG2b κ RYBP antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 89-148 mapping within an internal region of RYBP of human origin
- RYBP Antibody (A-1) is recommended for detection of RYBP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and porcine
- Anti-RYBP Antibody (A-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-374235 X, 200 µg/0.1 ml)
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RYBP Antibody (A-1) for WB and IHC(P) applications. These reagents are now offered in bundles with RYBP Antibody (A-1) (see ordering information below).
QUICK LINKS
SEE ALSO...
RYBP Antibody (A-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects RYBP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-RYBP antibody (A-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RYBP, or RING1- and YY1-binding protein, plays a crucial role in regulating gene expression, particularly in developmental processes, by being a key component of the mammalian polycomb complex. RYBP protein is essential for maintaining transcriptional repression of important developmental genes, including homeotic genes, which are vital for proper organismal development. RYBP′s interaction with E2F2 and E2F3 family members is particularly significant, as RYBP relies on the marked box domain within these proteins to stimulate Cdc6 promoter activity synergistically during the G1/S phase of the cell cycle. RYBP protein forms complexes with RING1 proteins and M33, which are integral to the polycomb group′s function in gene silencing. Widespread expression of RYBP, especially in lymphoid tissues and the placenta, underscores RYBP′s importance in various biological processes and highlights RYBP′s potential as a target for research in developmental biology and cancer studies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RYBP Antibody (A-1) References:
- RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1. | García, E., et al. 1999. EMBO J. 18: 3404-18. PMID: 10369680
- The death effector domain-associated factor plays distinct regulatory roles in the nucleus and cytoplasm. | Zheng, L., et al. 2001. J Biol Chem. 276: 31945-52. PMID: 11395500
- YEAF1/RYBP and YAF-2 are functionally distinct members of a cofactor family for the YY1 and E4TF1/hGABP transcription factors. | Sawa, C., et al. 2002. J Biol Chem. 277: 22484-90. PMID: 11953439
- Interaction of YY1 with E2Fs, mediated by RYBP, provides a mechanism for specificity of E2F function. | Schlisio, S., et al. 2002. EMBO J. 21: 5775-86. PMID: 12411495
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RYBP Antibody (A-1) | sc-374235 | 200 µg/ml | $322.00 | |||
RYBP Antibody (A-1): m-IgGκ BP-HRP Bundle | sc-522670 | 200 µg Ab, 40 µg BP | $361.00 | |||
RYBP Antibody (A-1): m-IgG2b BP-HRP Bundle | sc-549054 | 200 µg Ab; 10 µg BP | $361.00 | |||
RYBP Antibody (A-1) X | sc-374235 X | 200 µg/0.1 ml | $322.00 | |||
RYBP Antibody (A-1) AC | sc-374235 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RYBP Antibody (A-1) HRP | sc-374235 HRP | 200 µg/ml | $322.00 | |||
RYBP Antibody (A-1) FITC | sc-374235 FITC | 200 µg/ml | $336.00 | |||
RYBP Antibody (A-1) PE | sc-374235 PE | 200 µg/ml | $349.00 | |||
RYBP Antibody (A-1) Alexa Fluor® 488 | sc-374235 AF488 | 200 µg/ml | $364.00 | |||
RYBP Antibody (A-1) Alexa Fluor® 546 | sc-374235 AF546 | 200 µg/ml | $364.00 | |||
RYBP Antibody (A-1) Alexa Fluor® 594 | sc-374235 AF594 | 200 µg/ml | $364.00 | |||
RYBP Antibody (A-1) Alexa Fluor® 647 | sc-374235 AF647 | 200 µg/ml | $364.00 | |||
RYBP Antibody (A-1) Alexa Fluor® 680 | sc-374235 AF680 | 200 µg/ml | $364.00 | |||
RYBP Antibody (A-1) Alexa Fluor® 790 | sc-374235 AF790 | 200 µg/ml | $364.00 |