RPE Antibody (F-10): sc-393655
- RPE Antibody (F-10) is a mouse monoclonal IgG1 κ RPE antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 67-86 within an internal region of RPE of human origin
- RPE Antibody (F-10) is recommended for detection of RPE of mouse, rat and human origin and rcRPE of human origin by WB, IP, IF and ELISA
- Anti-RPE Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RPE Antibody (F-10) for WB applications. These reagents are now offered in bundles with RPE Antibody (F-10) (see ordering information below).
QUICK LINKS
SEE ALSO...
RPE Antibody (F-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects RPE protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-RPE antibody (F-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RPE protein, or ribulose-5-phosphate-3-epimerase, is a crucial enzyme in the pentose phosphate pathway, which plays a vital role in cellular metabolism by generating ribose-5-phosphate for nucleotide synthesis and NADPH for reductive biosynthesis. RPE protein consists of 228 amino acids and belongs to the ribulose phosphate binding superfamily, showing highest expression levels in red blood cells, lymphocytes, and fibroblasts. RPE exists as two alternatively spliced isoforms and shares 96% sequence identity with human protein rcRPE, being encoded by a gene located on human chromosome 2. This chromosome houses over 1,400 genes, accounting for nearly 8% of the human genome, and is associated with various genetic disorders, including Harlequin ichthyosis and Alström syndrome, which highlight RPE′s importance in both normal physiology and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RPE Antibody (F-10) References:
- Initiation sites for human DNA replication at a putative ribulose-5-phosphate 3-epimerase gene. | Hu, L., et al. 2004. Biochem Biophys Res Commun. 320: 648-55. PMID: 15240097
- Evidence for an ancestral alphoid domain on the long arm of human chromosome 2. | Avarello, R., et al. 1992. Hum Genet. 89: 247-9. PMID: 1587535
- D-Ribulose 5-phosphate 3-epimerase: functional and structural relationships to members of the ribulose-phosphate binding (beta/alpha)8-barrel superfamily. | Akana, J., et al. 2006. Biochemistry. 45: 2493-503. PMID: 16489742
- Origin of human chromosome 2: an ancestral telomere-telomere fusion. | IJdo, JW., et al. 1991. Proc Natl Acad Sci U S A. 88: 9051-5. PMID: 1924367
- Deletion 2q31.3----2q33.3: gene dosage effect of ribulose 5-phosphate 3-epimerase. | Dallapiccola, B., et al. 1988. Hum Genet. 79: 92. PMID: 3366467
- Purification, properties and assay of D-ribulose 5-phosphate 3-epimerase from human erythrocytes. | Karmali, A., et al. 1983. Biochem J. 211: 617-23. PMID: 6882362
- Biochemical genetics of the pentose phosphate cycle: human ribose 5-phosphate isomerase (RPI) and ribulose 5-phosphate 3-epimerase (RPE). | Spencer, N. and Hopkinson, DA. 1980. Ann Hum Genet. 43: 335-42. PMID: 7396409
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RPE Antibody (F-10) | sc-393655 | 200 µg/ml | $316.00 | |||
RPE Antibody (F-10): m-IgG Fc BP-HRP Bundle | sc-530528 | 200 µg Ab; 10 µg BP | $354.00 | |||
RPE Antibody (F-10): m-IgGκ BP-HRP Bundle | sc-524034 | 200 µg Ab, 40 µg BP | $354.00 | |||
RPE Antibody (F-10): m-IgG1 BP-HRP Bundle | sc-544108 | 200 µg Ab; 20 µg BP | $354.00 | |||
RPE Antibody (F-10) AC | sc-393655 AC | 500 µg/ml, 25% agarose | $416.00 | |||
RPE Antibody (F-10) HRP | sc-393655 HRP | 200 µg/ml | $316.00 | |||
RPE Antibody (F-10) FITC | sc-393655 FITC | 200 µg/ml | $330.00 | |||
RPE Antibody (F-10) PE | sc-393655 PE | 200 µg/ml | $343.00 | |||
RPE Antibody (F-10) Alexa Fluor® 488 | sc-393655 AF488 | 200 µg/ml | $357.00 | |||
RPE Antibody (F-10) Alexa Fluor® 546 | sc-393655 AF546 | 200 µg/ml | $357.00 | |||
RPE Antibody (F-10) Alexa Fluor® 594 | sc-393655 AF594 | 200 µg/ml | $357.00 | |||
RPE Antibody (F-10) Alexa Fluor® 647 | sc-393655 AF647 | 200 µg/ml | $357.00 | |||
RPE Antibody (F-10) Alexa Fluor® 680 | sc-393655 AF680 | 200 µg/ml | $357.00 | |||
RPE Antibody (F-10) Alexa Fluor® 790 | sc-393655 AF790 | 200 µg/ml | $357.00 | |||
RPE (F-10) Neutralizing Peptide | sc-393655 P | 100 µg/0.5 ml | $68.00 |