RPA40 Antibody (H-6): sc-374443
- RPA40 Antibody (H-6) is a mouse monoclonal IgG1 κ RPA40 antibody, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 1-105 of RPA40 of human origin
- RPA40 Antibody (H-6) is recommended for detection of RPA40 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-RPA40 Antibody (H-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RPA40 (H-6): sc-374443.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for RPA40 Antibody (H-6) for WB and IHC(P) applications. These reagents are now offered in bundles with RPA40 Antibody (H-6) (see ordering information below).
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RPA40 Antibody (H-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects RPA40 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RPA40 (H-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RPA40 plays a crucial role in ribosomal RNA transcription, essential for ribosome biogenesis and protein synthesis in cells. Located in the nucleolus, RPA40 functions as a vital subunit of RNA polymerase I (Pol I), responsible for transcribing class I genes that encode large ribosomal RNA. RPA40′s interaction with other subunits, such as RPA16 and RPA135, proves critical for Pol I complex assembly and functionality, highlighting RPA40′s importance in regulating ribosomal RNA synthesis in response to various physiological stimuli. RPA40 also serves as an essential component of RNA polymerase III (Pol III), further underscoring RPA40′s significance in cellular transcriptional machinery.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RPA40 Antibody (H-6) References:
- Human Nopp140, which interacts with RNA polymerase I: implications for rRNA gene transcription and nucleolar structural organization. | Chen, HK., et al. 1999. Mol Cell Biol. 19: 8536-46. PMID: 10567578
- Brain RNA polymerase and nucleolar structure in perinatal asphyxia of the rat. | Mosgoeller, W., et al. 2000. Exp Neurol. 161: 174-82. PMID: 10683283
- An approach for isolation of mutants defective in 35S ribosomal RNA synthesis in Saccharomyces cerevisiae. | Nogi, Y., et al. 1991. Proc Natl Acad Sci U S A. 88: 7026-30. PMID: 1871118
- Mouse RNA polymerase I 16-kDa subunit able to associate with 40-kDa subunit is a homolog of yeast AC19 subunit of RNA polymerases I and III. | Yao, Y., et al. 1996. J Biol Chem. 271: 32881-5. PMID: 8955128
- Molecular cloning and characterization of the cDNA encoding the largest subunit of mouse RNA polymerase I. | Seither, P., et al. 1997. Mol Gen Genet. 255: 180-6. PMID: 9236775
- Deficient transcription of subunit RPA 40 of RNA polymerase I and III in heart of rats with neonatal asphyxia. | Hoeger, H., et al. 1998. Life Sci. 62: 275-82. PMID: 9450498
- Regulation of mammalian ribosomal gene transcription by RNA polymerase I. | Grummt, I. 1999. Prog Nucleic Acid Res Mol Biol. 62: 109-54. PMID: 9932453
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RPA40 Antibody (H-6) | sc-374443 | 200 µg/ml | $316.00 | |||
RPA40 Antibody (H-6): m-IgG Fc BP-HRP Bundle | sc-527465 | 200 µg Ab; 10 µg BP | $354.00 | |||
RPA40 Antibody (H-6): m-IgG1 BP-HRP Bundle | sc-532838 | 200 µg Ab; 20 µg BP | $354.00 | |||
RPA40 Antibody (H-6) AC | sc-374443 AC | 500 µg/ml, 25% agarose | $416.00 | |||
RPA40 Antibody (H-6) HRP | sc-374443 HRP | 200 µg/ml | $316.00 | |||
RPA40 Antibody (H-6) FITC | sc-374443 FITC | 200 µg/ml | $330.00 | |||
RPA40 Antibody (H-6) PE | sc-374443 PE | 200 µg/ml | $343.00 | |||
RPA40 Antibody (H-6) Alexa Fluor® 488 | sc-374443 AF488 | 200 µg/ml | $357.00 | |||
RPA40 Antibody (H-6) Alexa Fluor® 546 | sc-374443 AF546 | 200 µg/ml | $357.00 | |||
RPA40 Antibody (H-6) Alexa Fluor® 594 | sc-374443 AF594 | 200 µg/ml | $357.00 | |||
RPA40 Antibody (H-6) Alexa Fluor® 647 | sc-374443 AF647 | 200 µg/ml | $357.00 | |||
RPA40 Antibody (H-6) Alexa Fluor® 680 | sc-374443 AF680 | 200 µg/ml | $357.00 | |||
RPA40 Antibody (H-6) Alexa Fluor® 790 | sc-374443 AF790 | 200 µg/ml | $357.00 |