RPA194 Antibody (C-1): sc-48385

RPA194 Antibody (C-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects RPA194 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RPA194 (C-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RPA194 protein, also known as polymerase (RNA) I polypeptide A, plays a crucial role in ribosomal RNA transcription, which is essential for ribosome biogenesis and, consequently, protein synthesis in cells. Located in the nucleolus, RNA polymerase I, of which RPA194 is the largest subunit, is responsible for transcribing class I genes that encode large ribosomal RNA. Proper functioning of RPA194 is vital, as RPA194 interacts with other subunits like RPA40 and RPA16, which are necessary for the assembly and integrity of the RNA polymerase I complex. Disruptions in RPA194 function can lead to impaired ribosomal RNA synthesis, affecting cellular growth and proliferation. Anti-RPA194 antibody (C-1) is an invaluable tool for researchers studying the mechanisms of ribosome biogenesis and the regulation of RNA polymerase I activity in various biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RPA194 Antibody (C-1) References:

1. Human Nopp140, which interacts with RNA polymerase I: implications for rRNA gene transcription and nucleolar structural organization.  |  Chen, HK., et al. 1999. Mol Cell Biol. 19: 8536-46. PMID: 10567578
2. Brain RNA polymerase and nucleolar structure in perinatal asphyxia of the rat.  |  Mosgoeller, W., et al. 2000. Exp Neurol. 161: 174-82. PMID: 10683283
3. An approach for isolation of mutants defective in 35S ribosomal RNA synthesis in Saccharomyces cerevisiae.  |  Nogi, Y., et al. 1991. Proc Natl Acad Sci U S A. 88: 7026-30. PMID: 1871118
4. A targeting modality for destruction of RNA polymerase I that possesses anticancer activity.  |  Peltonen, K., et al. 2014. Cancer Cell. 25: 77-90. PMID: 24434211
5. Severe neurodegenerative disease in brothers with homozygous mutation in POLR1A.  |  Kara, B., et al. 2017. Eur J Hum Genet. 25: 315-323. PMID: 28051070
6. HERC2 inactivation abrogates nucleolar localization of RecQ helicases BLM and WRN.  |  Zhu, M., et al. 2021. Sci Rep. 11: 360. PMID: 33432007
7. The natural history of progressive myoclonus ataxia.  |  van der Veen, S., et al. 2024. Neurobiol Dis. 199: 106555. PMID: 38844245
8. A role for the kinetochore protein, NUF2, in ribosome biogenesis.  |  Brown, TJ., et al. 2024. Mol Biol Cell. mbcE24080337. PMID: 39705402
9. Mouse RNA polymerase I 16-kDa subunit able to associate with 40-kDa subunit is a homolog of yeast AC19 subunit of RNA polymerases I and III.  |  Yao, Y., et al. 1996. J Biol Chem. 271: 32881-5. PMID: 8955128
10. Molecular cloning and characterization of the cDNA encoding the largest subunit of mouse RNA polymerase I.  |  Seither, P., et al. 1997. Mol Gen Genet. 255: 180-6. PMID: 9236775
11. Deficient transcription of subunit RPA 40 of RNA polymerase I and III in heart of rats with neonatal asphyxia.  |  Hoeger, H., et al. 1998. Life Sci. 62: 275-82. PMID: 9450498
12. Regulation of mammalian ribosomal gene transcription by RNA polymerase I.  |  Grummt, I. 1999. Prog Nucleic Acid Res Mol Biol. 62: 109-54. PMID: 9932453

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RPA194 Antibody (C-1) has 97 citations in a variety of scientific publications.

Primary antibodies like Anti-RPA194 Antibody (C-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RPA194 Antibody (C-1) Specifications

Alternate Product Name: RNA polymerase I subunit A

Product Clone Name: C-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: RPA194

Epitope Species: human

Product Reactivity/Detection: RPA194

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 97 citations in various scientific publications

Available Conjugates/Forms for RPA194 Antibody (C-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin