RPA16 Antibody (2774C3a): sc-81636

RPA16 Antibody (2774C3a) is a mouse monoclonal IgG1 antibody that detects RPA16 protein of human origin by western blotting (WB) and immunoprecipitation (IP). Anti-RPA16 antibody (2774C3a) is available as a non-conjugated format, providing researchers with a reliable tool for studying ribosomal biogenesis. RPA16 protein is a crucial component of RNA polymerase I (Pol I), which is primarily located in the nucleolus and is responsible for transcribing ribosomal RNA genes. This process is vital for the production of ribosomes, which are essential for protein synthesis in all living cells. The assembly of Pol I involves multiple subunits, including RPA16, RPA40, and RPA135, which interact at early stages of the complex formation. RPA40 not only plays a critical role in the stability and function of the Pol I complex but is also an essential subunit of RNA polymerase III, highlighting the interconnectedness of these transcriptional machinery components. Understanding the dynamics of RPA16 and its interactions within the Pol I complex is important for elucidating the regulatory mechanisms that govern ribosomal RNA synthesis and, consequently, cellular growth and proliferation.

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RPA16 Antibody (2774C3a) References:

1. Human Nopp140, which interacts with RNA polymerase I: implications for rRNA gene transcription and nucleolar structural organization.  |  Chen, HK., et al. 1999. Mol Cell Biol. 19: 8536-46. PMID: 10567578
2. Brain RNA polymerase and nucleolar structure in perinatal asphyxia of the rat.  |  Mosgoeller, W., et al. 2000. Exp Neurol. 161: 174-82. PMID: 10683283
3. An approach for isolation of mutants defective in 35S ribosomal RNA synthesis in Saccharomyces cerevisiae.  |  Nogi, Y., et al. 1991. Proc Natl Acad Sci U S A. 88: 7026-30. PMID: 1871118
4. Mouse RNA polymerase I 16-kDa subunit able to associate with 40-kDa subunit is a homolog of yeast AC19 subunit of RNA polymerases I and III.  |  Yao, Y., et al. 1996. J Biol Chem. 271: 32881-5. PMID: 8955128
5. Molecular cloning and characterization of the cDNA encoding the largest subunit of mouse RNA polymerase I.  |  Seither, P., et al. 1997. Mol Gen Genet. 255: 180-6. PMID: 9236775
6. Deficient transcription of subunit RPA 40 of RNA polymerase I and III in heart of rats with neonatal asphyxia.  |  Hoeger, H., et al. 1998. Life Sci. 62: 275-82. PMID: 9450498
7. Regulation of mammalian ribosomal gene transcription by RNA polymerase I.  |  Grummt, I. 1999. Prog Nucleic Acid Res Mol Biol. 62: 109-54. PMID: 9932453

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RPA16 Antibody (2774C3a) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-RPA16 Antibody (2774C3a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RPA16 Antibody (2774C3a) Specifications

Alternate Product Name: RPA16

Product Clone Name: 2774C3a

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: RPA16

Epitope Species: human

Product Reactivity/Detection: RPA16

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for RPA16 Antibody (2774C3a): N/A