RPA16 Activators
RPA16 activators belong to a specialized category of compounds that specifically interact with the Replication Protein A 16 kDa subunit, known as RPA16, which is a part of the larger RPA complex involved in DNA metabolism. Unlike inhibitors, activators are designed to enhance or stabilize the function of RPA16, which can have various effects on the protein's role in cellular processes. The RPA complex, including RPA16, is pivotal in DNA replication, repair, and recombination, and activators may affect these processes by promoting the proper assembly and stability of the complex, or by increasing the affinity of RPA for DNA. The chemical structures of these activators are often designed to interact with the RPA16 subunit in a way that facilitates its interaction with other subunits or with DNA, potentially leading to an upregulation of its normal function.
The development of RPA16 activators necessitates a deep understanding of the protein's biochemistry and the interactions within the RPA complex. Research into RPA16 activators often employs a multidisciplinary approach, incorporating techniques from molecular biology, structural biology, and chemistry to discover and refine molecules that can positively influence RPA16 activity. Detailed studies may include the use of X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, or cryo-electron microscopy to determine the three-dimensional structure of RPA16 in complex with activators. Additionally, biochemical assays are utilized to measure the effects of these activators on the functional dynamics of RPA16.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Aphidicolin | 38966-21-1 | sc-201535 sc-201535A sc-201535B | 1 mg 5 mg 25 mg | $84.00 $306.00 $1104.00 | 30 | |
A tetracyclic diterpene that acts as a specific inhibitor of DNA polymerase alpha and delta. Its action induces DNA replication stress, which can lead to the activation of the RPA complex as part of the DNA damage response. | ||||||
Hydroxyurea | 127-07-1 | sc-29061 sc-29061A | 5 g 25 g | $78.00 $260.00 | 18 | |
An agent that inhibits ribonucleotide reductase, resulting in decreased deoxyribonucleotide levels and subsequent activation of DNA damage checkpoints, potentially leading to RPA complex activation. | ||||||
Etoposide (VP-16) | 33419-42-0 | sc-3512B sc-3512 sc-3512A | 10 mg 100 mg 500 mg | $51.00 $231.00 $523.00 | 63 | |
A topoisomerase II inhibitor that induces DNA double-strand breaks and can lead to the activation of RPA as part of the cellular response to DNA damage. | ||||||
Cisplatin | 15663-27-1 | sc-200896 sc-200896A | 100 mg 500 mg | $138.00 $380.00 | 101 | |
A chemotherapy drug that forms DNA adducts and crosslinks, resulting in the recruitment and activation of DNA repair proteins, including RPA. | ||||||
L-Mimosine | 500-44-7 | sc-201536A sc-201536B sc-201536 sc-201536C | 25 mg 100 mg 500 mg 1 g | $36.00 $88.00 $220.00 $436.00 | 8 | |
An amino acid that can deplete deoxyribonucleotide pools and induce DNA replication stress, potentially leading to increased RPA activity. | ||||||
VE 821 | 1232410-49-9 | sc-475878 | 10 mg | $360.00 | ||
An inhibitor of the ATR kinase, which is involved in DNA damage response. Inhibition of ATR can enhance replication stress, requiring increased RPA complex activity for DNA repair. | ||||||
UCN-01 | 112953-11-4 | sc-202376 | 500 µg | $251.00 | 10 | |
A protein kinase inhibitor that can enhance the replication stress response, thereby potentially increasing the recruitment of RPA to sites of DNA damage. | ||||||
Olaparib | 763113-22-0 | sc-302017 sc-302017A sc-302017B | 250 mg 500 mg 1 g | $210.00 $305.00 $495.00 | 10 | |
A PARP inhibitor used to exploit synthetic lethality in cancer cells, inducing replication stress which may lead to increased RPA complex activity. | ||||||
Caffeine | 58-08-2 | sc-202514 sc-202514A sc-202514B sc-202514C sc-202514D | 50 g 100 g 250 g 1 kg 5 kg | $33.00 $67.00 $97.00 $192.00 $775.00 | 13 | |
A known inhibitor of ATM and ATR kinases, potentially enhancing replication stress and subsequently the activity of the RPA complex. | ||||||
2′-Deoxy-2′,2′-difluorocytidine | 95058-81-4 | sc-275523 sc-275523A | 1 g 5 g | $56.00 $128.00 | ||
A nucleoside analog that can cause replication stress and DNA damage, potentially leading to enhanced RPA complex activity. | ||||||