RNase L Antibody (H-4): sc-74416
- RNase L Antibody (H-4) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 442-741 mapping at the C-terminus of RNase L of human origin
- recommended for detection of RNase L of human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for RNase L Antibody (H-4) for WB and IHC(P) applications. These reagents are now offered in bundles with RNase L Antibody (H-4) (see ordering information below).
QUICK LINKS
SEE ALSO...
RNase L Antibody (H-4) is a mouse monoclonal IgG2a antibody that detects RNase L in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RNase L plays a crucial role in the interferon-regulated 2-5A system, which is essential for mediating antiviral and antiproliferative responses. RNase L is primarily located in the cytoplasm, where 2-5A, a product of interferon-induced enzymes, triggers activation. The cytoplasmic localization of RNase L enables rapid response to viral infections by degrading viral RNA and limiting viral replication. Additionally, mutations in RNase L have been linked to increased prostate cancer risk, highlighting RNase L′s importance not only in antiviral defense but also in tumor suppression. The interaction of RNase L with various cellular pathways underscores RNase L′s potential as a therapeutic target, making anti-RNase L antibody (H-4) an invaluable tool for researchers studying immune responses and cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RNase L Antibody (H-4) References:
- RNase L Suppresses Androgen Receptor Signaling, Cell Migration and Matrix Metalloproteinase Activity in Prostate Cancer Cells. | Dayal, S., et al. 2017. Int J Mol Sci. 18: PMID: 28257035
- Human serum RNase-L level is inversely associated with metabolic syndrome and age. | Wang, YT., et al. 2017. Cardiovasc Diabetol. 16: 46. PMID: 28399925
- OAS-RNase L innate immune pathway mediates the cytotoxicity of a DNA-demethylating drug. | Banerjee, S., et al. 2019. Proc Natl Acad Sci U S A. 116: 5071-5076. PMID: 30814222
- RNase L Antiviral Activity Is Not a Critical Component of the Oas1b-Mediated Flavivirus Resistance Phenotype. | Madden, JC., et al. 2019. J Virol. 93: PMID: 31462564
- RNase L promotes the formation of unique ribonucleoprotein granules distinct from stress granules. | Burke, JM., et al. 2020. J Biol Chem. 295: 1426-1438. PMID: 31896577
- The function of RNase L and its degradation mechanism in cardiac acute ischemic injury. | Ma, Q., et al. 2020. Apoptosis. 25: 400-411. PMID: 32385693
- Human Melanoma Cells Differentially Express RNASEL/RNase-L and miR-146a-5p under Sex Hormonal Stimulation. | Orlandi, E., et al. 2022. Curr Issues Mol Biol. 44: 4790-4802. PMID: 36286041
- Rational design and evaluation of 2-((pyrrol-2-yl)methylene)thiophen-4-ones as RNase L inhibitors. | Hwang, J., et al. 2023. Eur J Med Chem. 256: 115439. PMID: 37201427
- Origin of the OAS-RNase L innate immune pathway before the rise of jawed vertebrates via molecular tinkering. | Chu, L., et al. 2023. Proc Natl Acad Sci U S A. 120: e2304687120. PMID: 37487089
- A CRISPR-Cas9 knockout screening identifies IRF2 as a key driver of OAS3/RNase L-mediated RNA decay during viral infection. | Oh, S., et al. 2024. Proc Natl Acad Sci U S A. 121: e2412725121. PMID: 39475651
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNase L Antibody (H-4) | sc-74416 | 200 µg/ml | $316.00 | |||
RNase L Antibody (H-4): m-IgG Fc BP-HRP Bundle | sc-539514 | 200 µg Ab; 10 µg BP | $354.00 | |||
RNase L Antibody (H-4): m-IgG2a BP-HRP Bundle | sc-546719 | 200 µg Ab; 10 µg BP | $354.00 |