RNase III Drosha Antibody (C-7): sc-393591

RNase III Drosha Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects RNase III Drosha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RNase III Drosha monoclonal antibody (C-7) is raised against amino acids 1071-1370 mapping at the C-terminus of RNase III of human origin. RNase III Drosha (C-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RNase III Drosha plays a critical role in microRNA biogenesis, essential regulators of gene expression. Located primarily in the nucleus, RNase III Drosha processes primary microRNA transcripts (pri-miRNAs) into precursor microRNAs (pre-miRNAs), a crucial step in microRNA maturation. This processing regulates gene expression, as microRNAs inhibit target mRNAs, influencing cellular processes like development, differentiation, and stress response. RNase III Drosha functions within a multi-protein complex including DGCR8, enhancing specificity and efficiency in recognizing and cleaving pri-miRNAs. RNase III Drosha and DGCR8 interaction demonstrates how protein-protein interactions regulate RNA processing, revealing complex mechanisms governing gene expression and cellular function.

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RNase III Drosha Antibody (C-7) References:

1. Mutational analysis of the nuclease domain of Escherichia coli ribonuclease III. Identification of conserved acidic residues that are important for catalytic function in vitro.  |  Sun, W., et al. 2004. Biochemistry. 43: 13054-62. PMID: 15476399
2. The Microprocessor complex mediates the genesis of microRNAs.  |  Gregory, RI., et al. 2004. Nature. 432: 235-40. PMID: 15531877
3. Processing of primary microRNAs by the Microprocessor complex.  |  Denli, AM., et al. 2004. Nature. 432: 231-5. PMID: 15531879
4. Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha.  |  Zeng, Y., et al. 2005. EMBO J. 24: 138-48. PMID: 15565168
5. The Drosha-DGCR8 complex in primary microRNA processing.  |  Han, J., et al. 2004. Genes Dev. 18: 3016-27. PMID: 15574589
6. The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis.  |  Landthaler, M., et al. 2004. Curr Biol. 14: 2162-7. PMID: 15589161
7. MicroRNA biogenesis: drosha can't cut it without a partner.  |  Tomari, Y. and Zamore, PD. 2005. Curr Biol. 15: R61-4. PMID: 15668159

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RNase III Drosha Antibody (C-7) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-RNase III Drosha Antibody (C-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RNase III Drosha Antibody (C-7) Specifications

Alternate Product Name: Drosha Ribonuclease III

Product Clone Name: C-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1071-1370

Epitope Target: RNase III

Epitope Species: human

Product Reactivity/Detection: RNase III Drosha isoforms 1 and 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for RNase III Drosha Antibody (C-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin