RLF Antibody (D-9): sc-166128

RLF Antibody (D-9) is a mouse monoclonal IgG1 antibody that detects RLF in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RLF, or Ras-associated protein Rab2L/RalGDS-like factor, plays a crucial role as a guanine nucleotide exchange factor (GEF) for the small GTPase Ral, acting as a key intermediary in the Ras signaling pathway. RLF is primarily located in the cytoplasm and is essential for mediating Ras-dependent signaling, which is vital for cellular processes such as proliferation and differentiation. RLF interaction with the GTP-bound form of Ras proteins through its C-terminal Ras-binding domain facilitates the activation of Ral, leading to the phosphorylation of c-Jun at Serines 63 and 73. This phosphorylation is critical for the transcriptional regulation of genes involved in cell growth and transformation, highlighting RLF′s importance in oncogenic signaling pathways. Additionally, RLF′s ability to induce transcriptional activation and promote cell growth, particularly when targeted to the plasma membrane, underscores RLF significance in cellular signaling and potential implications in cancer biology. Anti-RLF antibody (D-9) is an invaluable tool for researchers studying the intricate mechanisms of Ras signaling and its impact on cellular behavior.

The preferred RLF antibody is RLF Antibody (D-7): sc-365373.