RKIP Antibody (D-5): sc-365973
- RKIP Antibody (D-5) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 143-174 near the C-terminus of RKIP of rat origin
- recommended for detection of RKIP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RKIP Antibody (D-5) for WB and IHC(P) applications. These reagents are now offered in bundles with RKIP Antibody (D-5) (see ordering information below).
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RKIP Antibody (D-5) is a mouse monoclonal IgG1 antibody that detects Raf kinase inhibitor protein (RKIP) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). RKIP is a crucial cytosolic protein that plays a significant role in regulating the MAP kinase signaling pathway, which is vital for cell proliferation, differentiation, and survival. RKIP (D-5) antibody targets a protein primarily located in the cytoplasm, where RKIP interacts with the serine/threonine kinase Raf-1, inhibiting Raf-1 activity and thereby modulating downstream signaling events. This regulation is particularly important as RKIP prevents excessive activation of MEK, a key substrate of Raf-1, which in turn reduces the activation of ERK1 and ERK2, ultimately influencing AP-1 mediated transcription. The ability of RKIP to bind both active and inactive forms of Raf-1 highlights RKIP′s role as a critical regulator of cellular signaling, making anti-RKIP antibody (D-5) an essential tool for researchers studying the intricacies of MAP kinase pathway modulation and its implications in various diseases, including cancer.
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RKIP Antibody (D-5) References:
- Raf kinase inhibitory protein (RKIP): a physiological regulator and future therapeutic target. | Zeng, L., et al. 2008. Expert Opin Ther Targets. 12: 1275-87. PMID: 18781826
- Raf kinase inhibitor protein RKIP enhances signaling by glycogen synthase kinase-3β. | Al-Mulla, F., et al. 2011. Cancer Res. 71: 1334-43. PMID: 21303975
- Expression of Raf Kinase Inhibitor Protein (RKIP) is a predictor of uveal melanoma metastasis. | Caltabiano, R., et al. 2014. Histol Histopathol. 29: 1325-34. PMID: 24763848
- Raf kinase inhibitor protein mediated signaling inhibits invasion and metastasis of hepatocellular carcinoma. | Wu, X., et al. 2016. Biochim Biophys Acta. 1860: 384-91. PMID: 26116914
- Raf kinase inhibitor protein mediates intestinal epithelial cell apoptosis and promotes IBDs in humans and mice. | Lin, W., et al. 2017. Gut. 66: 597-610. PMID: 26801887
- Raf kinase inhibitor protein (RKIP) inhibits the cell migration and invasion in human glioma cell lines in vitro. | Lei, X., et al. 2015. Int J Clin Exp Pathol. 8: 14214-20. PMID: 26823735
- Raf kinase inhibitor protein regulates oxygen-glucose deprivation-induced PC12 cells apoptosis through the NF-κB and ERK pathways. | Su, L., et al. 2016. J Clin Biochem Nutr. 59: 86-92. PMID: 27698534
- Raf kinase inhibitor protein mediates myocardial fibrosis under conditions of enhanced myocardial oxidative stress. | Kazakov, A., et al. 2018. Basic Res Cardiol. 113: 42. PMID: 30191336
- Investigation of Marine-Derived Natural Products as Raf Kinase Inhibitory Protein (RKIP)-Binding Ligands. | Parate, S., et al. 2021. Mar Drugs. 19: PMID: 34677480
- Vicagrel is hydrolyzed by Raf kinase inhibitor protein in human intestine. | Zhu, T., et al. 2022. Biopharm Drug Dispos. 43: 247-254. PMID: 36519186
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RKIP Antibody (D-5) | sc-365973 | 200 µg/ml | $322.00 | |||
RKIP Antibody (D-5): m-IgG Fc BP-HRP Bundle | sc-537860 | 200 µg Ab; 10 µg BP | $361.00 | |||
RKIP Antibody (D-5): m-IgGκ BP-HRP Bundle | sc-535289 | 200 µg Ab; 40 µg BP | $361.00 | |||
RKIP Antibody (D-5): m-IgG1 BP-HRP Bundle | sc-545410 | 200 µg Ab; 20 µg BP | $361.00 | |||
RKIP (D-5) Neutralizing Peptide | sc-365973 P | 100 µg/0.5 ml | $69.00 |