RKIP Antibody (H-10): sc-376925

RKIP Antibody (H-10) is a mouse monoclonal IgG1 antibody that detects RKIP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RKIP (H-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Raf kinase inhibitor protein (RKIP) plays a crucial role in cellular signaling as a cytosolic protein that not only binds phosphatidylethanolamine but also interacts with key components of the MAP kinase pathway. RKIP′s ability to bind both active and inactive forms of Raf-1 allows regulation of the signaling cascade effectively, inhibiting the activation of the Raf-1 substrate MEK. Such inhibition leads to decreased activation of downstream MAP kinase signaling pathways, including those involving ERK1 and ERK2, which are essential for various cellular processes such as proliferation, differentiation, and survival. RKIP modulation of these pathways maintains cellular homeostasis and influences tumor progression and metastasis in cancer biology.

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RKIP Antibody (H-10) References:

1. Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP.  |  Yeung, K., et al. 1999. Nature. 401: 173-7. PMID: 10490027
2. Characterization of the Raf kinase inhibitory protein (RKIP) binding pocket: NMR-based screening identifies small-molecule ligands.  |  Shemon, AN., et al. 2010. PLoS One. 5: e10479. PMID: 20463977
3. Expression of phosphorylated raf kinase inhibitor protein (pRKIP) is a predictor of lung cancer survival.  |  Huerta-Yepez, S., et al. 2011. BMC Cancer. 11: 259. PMID: 21689459
4. Raf kinase inhibitor protein (RKIP) deficiency decreases latency of tumorigenesis and increases metastasis in a murine genetic model of prostate cancer.  |  Escara-Wilke, J., et al. 2015. Prostate. 75: 292-302. PMID: 25327941
5. Raf kinase inhibitor protein regulates oxygen-glucose deprivation-induced PC12 cells apoptosis through the NF-κB and ERK pathways.  |  Su, L., et al. 2016. J Clin Biochem Nutr. 59: 86-92. PMID: 27698534
6. Raf Kinase Inhibitor Protein (RKIP) Inhibits Tumor Necrosis Factor-α (TNF-α) Induced Adhesion Molecules Expression in Vascular Smooth Muscle Bells by Suppressing (Nuclear Transcription Factor-κB (NF-kappaB) Pathway.  |  Jing, SH., et al. 2017. Med Sci Monit. 23: 4789-4797. PMID: 28983072
7. Cross-Talks between Raf Kinase Inhibitor Protein and Programmed Cell Death Ligand 1 Expressions in Cancer: Role in Immune Evasion and Therapeutic Implications.  |  Ho, M. and Bonavida, B. 2024. Cells. 13: PMID: 38786085
8. Sequence homology of rat and human HCNP precursor proteins, bovine phosphatidylethanolamine-binding protein and rat 23-kDa protein associated with the opioid-binding protein.  |  Tohdoh, N., et al. 1995. Brain Res Mol Brain Res. 30: 381-4. PMID: 7637590
9. Differential activation of ERK and JNK mitogen-activated protein kinases by Raf-1 and MEKK.  |  Minden, A., et al. 1994. Science. 266: 1719-23. PMID: 7992057
10. Sequence analysis of a mammalian phospholipid-binding protein from testis and epididymis and its distribution between spermatozoa and extracellular secretions.  |  Perry, AC., et al. 1994. Biochem J. 301 (Pt 1): 235-42. PMID: 8037677
11. Inhibition of Raf-1 signaling by a monoclonal antibody, which interferes with Raf-1 activation and with Mek substrate binding.  |  Kolch, W., et al. 1996. Oncogene. 13: 1305-14. PMID: 8808705
12. The complexity of Raf-1 regulation.  |  Morrison, DK. and Cutler, RE. 1997. Curr Opin Cell Biol. 9: 174-9. PMID: 9069260

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RKIP Antibody (H-10) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-RKIP Antibody (H-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RKIP Antibody (H-10) Specifications

Alternate Product Name: PEBP1

Product Clone Name: H-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 1-187

Epitope Target: RKIP

Epitope Species: human

Product Reactivity/Detection: RKIP and processed active peptide HCNP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for RKIP Antibody (H-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin