RKIP Antibody (8): sc-101504

RKIP Antibody (8) is a mouse monoclonal IgG2b antibody that detects Raf kinase inhibitor protein (RKIP) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RKIP plays a crucial role in regulating the MAP kinase signaling pathway, which is essential for various cellular processes such as proliferation, differentiation, and apoptosis. RKIP is primarily located in the cytosol, where RKIP interacts with key signaling molecules, including the serine/threonine kinase Raf-1. By binding to both active and inactive forms of Raf-1, RKIP modulates the activity of downstream effectors like MEK and ERK, thereby influencing cellular responses to growth factors and stress signals. RKIP′s ability to inhibit MEK activation by competing with its substrate is particularly important, as this mechanism helps maintain the balance of signaling pathways that can lead to oncogenic transformation when dysregulated. With versatile applications and significant role in cellular signaling, anti-RKIP antibody (8) serves as an invaluable tool for researchers studying MAP kinase pathway regulation and its implications in various diseases, including cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RKIP Antibody (8) References:

1. RAF-kinase inhibitor protein (RKIP) downregulation in esophageal cancer and its metastases.  |  Birner, P., et al. 2012. Clin Exp Metastasis. 29: 551-9. PMID: 22460832
2. Survey of Raf kinase inhibitor protein (RKIP) in multiple cancer types.  |  Lamiman, K., et al. 2014. Crit Rev Oncog. 19: 455-68. PMID: 25597355
3. Role of Raf kinase inhibitor protein in Helicobacter pylori-mediated signaling in gastric cancer.  |  Nisimova, L., et al. 2014. Crit Rev Oncog. 19: 469-81. PMID: 25597356
4. Human Intestinal Raf Kinase Inhibitor Protein (RKIP) Catalyzes Prasugrel as a Bioactivation Hydrolase.  |  Kazui, M., et al. 2016. Drug Metab Dispos. 44: 115-23. PMID: 26558823
5. Raf Kinase Inhibitor Protein Attenuates Ischemic-Induced Microglia Cell Apoptosis and Activation Through NF-κB Pathway.  |  Su, L., et al. 2017. Cell Physiol Biochem. 41: 1125-1134. PMID: 28245468
6. Crosstalks between Raf-kinase inhibitor protein and cancer stem cell transcription factors (Oct4, KLF4, Sox2, Nanog).  |  Lee, S., et al. 2017. Tumour Biol. 39: 1010428317692253. PMID: 28378634
7. Raf Kinase Inhibitor Protein Preferentially Promotes TLR3-Triggered Signaling and Inflammation.  |  Lai, R., et al. 2017. J Immunol. 198: 4086-4095. PMID: 28411188
8. RAF Kinase Inhibitor Protein in Myeloid Leukemogenesis.  |  Zebisch, A., et al. 2019. Int J Mol Sci. 20: PMID: 31744053
9. Mycobacterium tuberculosis Raf kinase inhibitor protein (RKIP) Rv2140c is involved in cell wall arabinogalactan biosynthesis via phosphorylation.  |  Abo-Kadoum, MA., et al. 2021. Microbiol Res. 242: 126615. PMID: 33189070
10. Raf kinase inhibitor protein combined with phosphorylated extracellular signal-regulated kinase offers valuable prognosis in gastrointestinal stromal tumor.  |  Qu, WZ., et al. 2023. World J Gastroenterol. 29: 4200-4213. PMID: 37475847

The preferred RKIP antibody is RKIP Antibody (H-10): sc-376925.