RKIP Antibody (8) recommended for detection of RKIP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

RKIP Antibody (8): sc-101504

RKIP Antibody (8) is rated 5.0 out of 5 by 5.
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Datasheets

    • RKIP Antibody (8) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against recombinant RKIP of human origin
    • recommended for detection of RKIP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

I am using RKIP (8): sc-101504 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 功能不错 Single strong band in WB in human transfected 293 whole cell lysates
Date published: 2019-03-16
Rated 5 out of 5 by from Great antibody Purchased the RKIP Antibody (8), and as received a sample of the secondary antibody, mouse IgG kappa - HRP. Strong signal is seen at the expected size (23 kDa) in MCF7 cells and other cell lines using the provided secondary.
Date published: 2016-12-13
Rated 5 out of 5 by from Band detected at expected mw in 293T and Band detected at expected mw in 293T and Hep G2 whole cell lysates. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmic Produced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human liver tissue. -SCBT QC
Date published: 2015-01-08
Rated 5 out of 5 by from WB publishable data with HNE1 and Huvec WB publishable data with HNE1 and Huvec - SCBT Publication Review
Date published: 2014-08-03
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