RGS12 Antibody (A-2): sc-514173

RGS12 Antibody (A-2) is a mouse monoclonal IgG2b antibody that detects RGS12 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RGS12 is a member of the regulators of G-protein signaling (RGS) family, which plays a crucial role in modulating G protein-coupled receptor signaling pathways. RGS12 functions as a GTPase-activating protein for Gi class alpha subunits, effectively shortening the lifespan of activated G proteins and fine-tuning cellular responses to hormones and neurotransmitters. RGS12 is characterized by a complex structure that includes a Ras-binding domain, PDZ and PTB domains, and unique "LGN motifs," which serve as guanine nucleotide exchange factors specific for G protein alpha-subunits. The human RGS12 gene, located on chromosome 4p16.3, produces twelve distinct transcripts through intricate splicing, with high expression levels in the brain and lung, and lower levels in tissues such as testis, heart, and spleen. The diverse expression and multifunctionality of RGS12 underscore its significance in various physiological processes, making anti-RGS12 antibody (A-2) an essential tool for researchers studying G protein signaling pathways and their implications in health and disease.

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RGS12 Antibody (A-2) References:

1. Mapping of RGS12-Cav2.2 channel interaction.  |  Richman, RW. and Diversé-Pierluissi, MA. 2004. Methods Enzymol. 390: 224-39. PMID: 15488181
2. RGS12 interacts with the SNARE-binding region of the Cav2.2 calcium channel.  |  Richman, RW., et al. 2005. J Biol Chem. 280: 1521-8. PMID: 15536086
3. RGS12 is essential for RANKL-evoked signaling for terminal differentiation of osteoclasts in vitro.  |  Yang, S. and Li, YP. 2007. J Bone Miner Res. 22: 45-54. PMID: 17042716
4. Regulator of G Protein Signaling Protein 12 (Rgs12) Controls Mouse Osteoblast Differentiation via Calcium Channel/Oscillation and Gαi-ERK Signaling.  |  Li, Z., et al. 2019. J Bone Miner Res. 34: 752-764. PMID: 30489658
5. Role of RGS12 in the differential regulation of kappa opioid receptor-dependent signaling and behavior.  |  Gross, JD., et al. 2019. Neuropsychopharmacology. 44: 1728-1741. PMID: 31141817
6. A role for Regulator of G protein Signaling-12 (RGS12) in the balance between myoblast proliferation and differentiation.  |  Schroer, AB., et al. 2019. PLoS One. 14: e0216167. PMID: 31408461
7. Genetic deletion of Rgs12 in mice affects serotonin transporter expression and function in vivo and ex vivo.  |  White, AN., et al. 2020. J Psychopharmacol. 34: 1393-1407. PMID: 32842837
8. RGS12 inhibits the progression and metastasis of multiple myeloma by driving M1 macrophage polarization and activation in the bone marrow microenvironment.  |  Yuan, G., et al. 2022. Cancer Commun (Lond). 42: 60-64. PMID: 34931491
9. Distinct and overlapping RGS14 and RGS12 actions regulate NPT2A-mediated phosphate transport.  |  Sneddon, WB., et al. 2024. Biochem Biophys Res Commun. 733: 150700. PMID: 39293332
10. RGS12 is a target of penehyclidine hydrochloride that enhances oxidative stress and ferroptosis in a model of myocardial ischemia/reperfusion injury by inhibiting the Nrf2 pathway.  |  Zi, C., et al. 2025. Int J Mol Med. 55: PMID: 39930821

The preferred RGS12 antibody is RGS12 Antibody (G-4): sc-398545.