RGS12 Antibody (G-4): sc-398545

RGS12 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects RGS12 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RGS12 (G-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RGS12 plays a crucial role in regulating G-protein signaling pathways, acting as a GTPase-activating protein specifically for Gi class alpha subunits. This function helps modulate the duration and intensity of signaling initiated by hormones and neurotransmitters, thereby influencing numerous physiological processes such as cardiac function and neuronal signaling. RGS12 is characterized by a complex structure, including a Ras-binding domain, PDZ and PTB domains, and unique "LGN motifs" that serve as guanine nucleotide exchange factors for G protein alpha-subunits. The human RGS12 gene, located on chromosome 4p16.3, undergoes intricate splicing to produce twelve distinct transcripts, predominantly expressed in the brain and lung, with lower expression levels in the testis, heart, and spleen. This tissue-specific expression and diverse functional roles of RGS12 underscore its importance in cellular signaling and potential implications in various diseases, making anti-RGS12 antibody (G-4) an essential tool for researchers studying G-protein signaling pathways.

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RGS12 Antibody (G-4) References:

1. Emerging roles for RGS proteins in cell signalling.  |  Hepler, JR. 1999. Trends Pharmacol Sci. 20: 376-82. PMID: 10462761
2. Raf-like Ras/Rap-binding domains in RGS12- and still-life-like signalling proteins.  |  Ponting, CP. 1999. J Mol Med (Berl). 77: 695-8. PMID: 10606204
3. Novel alternative splicing and nuclear localization of human RGS12 gene products.  |  Chatterjee, TK. and Fisher, RA. 2000. J Biol Chem. 275: 29660-71. PMID: 10869340
4. RGS12 Is a Novel Critical NF-κB Activator in Inflammatory Arthritis.  |  Yuan, G., et al. 2020. iScience. 23: 101172. PMID: 32512384
5. RGS12 is a novel tumor suppressor in osteosarcoma that inhibits YAP-TEAD1-Ezrin signaling.  |  Li, Y., et al. 2021. Oncogene. 40: 2553-2566. PMID: 33686240
6. RGS12 Drives Macrophage Activation and Osteoclastogenesis in Periodontitis.  |  Yuan, G., et al. 2022. J Dent Res. 101: 448-457. PMID: 34796776
7. Macrophage RGS12 contributes to osteoarthritis pathogenesis through enhancing the ubiquitination.  |  Yuan, G., et al. 2022. Genes Dis. 9: 1357-1367. PMID: 35873013
8. RGS12 represses oral squamous cell carcinoma by driving M1 polarization of tumor-associated macrophages via controlling ciliary MYCBP2/KIF2A signaling.  |  Yuan, G., et al. 2023. Int J Oral Sci. 15: 11. PMID: 36797232
9. Endothelial RGS12 governs angiogenesis in inflammatory arthritis by controlling cilia formation and elongation via MYCBP2 signaling.  |  Yuan, G., et al. 2022. Cell Insight. 1: 100055. PMID: 37193553
10. Molecular cloning and expression analysis of rat Rgs12 and Rgs14.  |  Snow, BE., et al. 1997. Biochem Biophys Res Commun. 233: 770-7. PMID: 9168931
11. GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/Dlg/ZO-1) domain.  |  Snow, BE., et al. 1998. J Biol Chem. 273: 17749-55. PMID: 9651375
12. Cardiac myocytes express mRNA for ten RGS proteins: changes in RGS mRNA expression in ventricular myocytes and cultured atria.  |  Kardestuncer, T., et al. 1998. FEBS Lett. 438: 285-8. PMID: 9827562

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RGS12 Antibody (G-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-RGS12 Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RGS12 Antibody (G-4) Specifications

Alternate Product Name: RGS12

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 3-302

Epitope Target: RGS12

Epitope Species: human

Product Reactivity/Detection: RGS12 isoforms 1 and 4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for RGS12 Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin