RCC1 Antibody (E-6): sc-55559

RCC1 Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects RCC1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). RCC1 (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RCC1, known as the regulator of chromosome condensation 1, plays a crucial role in regulating the small Ras-related protein Ran, which is essential for numerous cellular processes such as DNA replication, mitotic entry and exit, and the transport of RNA and proteins through the nuclear pore complex. RCC1 acts as a guanine nucleotide exchange factor (GEF) that facilitates the conversion of inactive Ran GDP to its active form, Ran GTP, thereby influencing the dynamics of nuclear transport and cell cycle progression. The balance between the active and inactive forms of Ran is tightly controlled by RCC1 and Ran GAP1, which enhances the hydrolysis of GTP to GDP, ensuring cellular processes are executed with precision. RCC1′s activity can be modulated by proteins such as Ran BP1, which inhibits RCC1, and Ran BP2, which serves as a docking site for Ran GTP at the nuclear pore complex, highlighting the intricate regulatory mechanisms that govern cellular function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RCC1 Antibody (E-6) References:

1. The Multifaceted Roles of RCC1 in Tumorigenesis.  |  Ren, X., et al. 2020. Front Mol Biosci. 7: 225. PMID: 33102517
2. RCC1 Expression as a Prognostic Marker in Colorectal Liver Oligometastases.  |  Deng, Y., et al. 2021. Pathol Oncol Res. 27: 1610077. PMID: 34924821
3. The intricate roles of RCC1 in normal cells and cancer cells.  |  Jing, L. and Kwok, HF. 2022. Biochem Soc Trans. 50: 83-93. PMID: 35191966
4. Synergistic effect of HDAC inhibitor Chidamide with Cladribine on cell cycle arrest and apoptosis by targeting HDAC2/c-Myc/RCC1 axis in acute myeloid leukemia.  |  Gu, S., et al. 2023. Exp Hematol Oncol. 12: 23. PMID: 36849955
5. RCC1 regulation of subcellular protein localization via Ran GTPase drives pancreatic ductal adenocarcinoma growth.  |  Bannoura, SF., et al. 2024. Cancer Lett. 604: 217275. PMID: 39321913
6. GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import.  |  Melchior, F., et al. 1995. J Cell Biol. 131: 571-81. PMID: 7593180
7. The Ran/TC4 GTPase-binding domain: identification by expression cloning and characterization of a conserved sequence motif.  |  Beddow, AL., et al. 1995. Proc Natl Acad Sci U S A. 92: 3328-32. PMID: 7724562
8. Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis.  |  Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
9. Interaction of the nuclear GTP-binding protein Ran with its regulatory proteins RCC1 and RanGAP1.  |  Klebe, C., et al. 1995. Biochemistry. 34: 639-47. PMID: 7819259
10. Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1.  |  Bischoff, FR., et al. 1995. EMBO J. 14: 705-15. PMID: 7882974
11. Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form.  |  Scheffzek, K., et al. 1995. Nature. 374: 378-81. PMID: 7885480
12. Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing.  |  Ren, M., et al. 1995. Mol Cell Biol. 15: 2117-24. PMID: 7891706

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RCC1 Antibody (E-6) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-RCC1 Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RCC1 Antibody (E-6) Specifications

Alternate Product Name: RCC1

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 122-421

Epitope Target: RCC1

Epitope Species: human

Product Reactivity/Detection: RCC1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for RCC1 Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin