RBP2 Antibody (G-12): sc-365993

RBP2 Antibody (G-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects RBP2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RBP2 (G-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RBP2, also known as Retinoblastoma-binding protein 2, RBBP2, JARID1A, or KDM5A, is a nuclear protein that plays a critical role in regulating gene expression and cell cycle progression. RBP2 functions as a histone demethylase, specifically targeting methyl residues on Histone H3, which is essential for modulating chromatin structure and gene accessibility. This enzymatic activity maintains proper expression of genes involved in cell proliferation and differentiation. RBP2′s interaction with retinoblastoma protein (Rb) and other regulatory proteins, such as rhombotin-2 (LMO2), underscores RBP2′s importance in cellular signaling pathways that govern cell cycle control. Multiple isoforms of RBP2, resulting from alternative splicing, add another layer of complexity to RBP2′s functional repertoire, allowing for diverse regulatory mechanisms in various cellular contexts.

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RBP2 Antibody (G-12) References:

1. Retinoblastoma-binding protein 2 (Rbp2) potentiates nuclear hormone receptor-mediated transcription.  |  Chan, SW. and Hong, W. 2001. J Biol Chem. 276: 28402-12. PMID: 11358960
2. Binding of pRB to the PHD protein RBP2 promotes cellular differentiation.  |  Benevolenskaya, EV., et al. 2005. Mol Cell. 18: 623-35. PMID: 15949438
3. Re-expression of the retinoblastoma-binding protein 2-homolog 1 reveals tumor-suppressive functions in highly metastatic melanoma cells.  |  Roesch, A., et al. 2006. J Invest Dermatol. 126: 1850-9. PMID: 16645588
4. RBP2 belongs to a family of demethylases, specific for tri-and dimethylated lysine 4 on histone 3.  |  Christensen, J., et al. 2007. Cell. 128: 1063-76. PMID: 17320161
5. The retinoblastoma binding protein RBP2 is an H3K4 demethylase.  |  Klose, RJ., et al. 2007. Cell. 128: 889-900. PMID: 17320163
6. RBP2 is an MRG15 complex component and down-regulates intragenic histone H3 lysine 4 methylation.  |  Hayakawa, T., et al. 2007. Genes Cells. 12: 811-26. PMID: 17573780
7. Cloning of cDNAs for cellular proteins that bind to the retinoblastoma gene product.  |  Defeo-Jones, D., et al. 1991. Nature. 352: 251-4. PMID: 1857421
8. Critical role of histone demethylase RBP2 in human gastric cancer angiogenesis.  |  Li, L., et al. 2014. Mol Cancer. 13: 81. PMID: 24716659
9. RBP2 Promotes Adult Acute Lymphoblastic Leukemia by Upregulating BCL2.  |  Wang, X., et al. 2016. PLoS One. 11: e0152142. PMID: 27008505
10. Role of RBP2-Induced ER and IGF1R-ErbB Signaling in Tamoxifen Resistance in Breast Cancer.  |  Choi, HJ., et al. 2018. J Natl Cancer Inst. 110: PMID: 29028222
11. Characterization of the retinoblastoma binding proteins RBP1 and RBP2.  |  Fattaey, AR., et al. 1993. Oncogene. 8: 3149-56. PMID: 8414517
12. T-cell oncogene rhombotin-2 interacts with retinoblastoma-binding protein 2.  |  Mao, S., et al. 1997. Oncogene. 14: 1531-9. PMID: 9129143

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RBP2 Antibody (G-12) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-RBP2 Antibody (G-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RBP2 Antibody (G-12) Specifications

Alternate Product Name: RBP2

Product Clone Name: G-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 801-900

Epitope Target: RBP2

Epitope Species: human

Product Reactivity/Detection: RBP2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for RBP2 Antibody (G-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin